Production of branched-chain aroma compounds by Propionibacterium freudenreichii: links with the biosynthesis of membrane fatty acids

Short branched-chain fatty acids (BCFAs) are cheese flavour compounds, which result from the conversion of branched-chain amino acids (BCAAs). In Swiss cheese, the production of short BCFAs is mainly performed by Propionibacterium freudenreichii and is strain dependent. Our aim was to investigate th...

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Bibliographic Details
Published in:Journal of applied microbiology 2008-10, Vol.105 (4), p.977-985
Main Authors: Dherbécourt, J, Maillard, M.-B, Catheline, D, Thierry, A
Format: Article
Language:English
Subjects:
Amino Acids, Branched-Chain
Amino Acids, Branched-Chain - analysis
Amino Acids, Branched-Chain - biosynthesis
Animals
aroma compounds
Bacteriological Techniques
Biological and medical sciences
branched-chain fatty acid
Cell Membrane
Cell Membrane - metabolism
Cheese
Cheese - microbiology
dairies
dairy
Fatty Acids
Fatty Acids - analysis
Fatty Acids - biosynthesis
Food Microbiology
Fundamental and applied biological sciences. Psychology
Humans
Leucine
Leucine - metabolism
Life Sciences
membrane
Microbiology
Microbiology and Parasitology
odor compounds
Propionibacterium
Propionibacterium - metabolism
Propionibacterium freudenreichii
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Summary:Short branched-chain fatty acids (BCFAs) are cheese flavour compounds, which result from the conversion of branched-chain amino acids (BCAAs). In Swiss cheese, the production of short BCFAs is mainly performed by Propionibacterium freudenreichii and is strain dependent. Our aim was to investigate the possible links between the biosynthesis of short BCFAs and membrane BCFAs in P. freudenreichii. Short and membrane BCFAs were analysed by gas chromatography-mass spectrometry. Two strains differing in their capacities to release short BCFAs were selected. Tri-deuterated-labelled leucine was used in both strains as a precursor of short extracellular iso-BCFAs and of membrane iso-BCFAs. The proportions of anteiso : iso BCFAs synthesized varied as function of the BCAAs provided in the growth medium, from 72 : 28 to 100 : 0, with leucine and valine, and with isoleucine as sole BC precursors, respectively. The branching pattern of short BCFAs exactly matched that of membrane BCFAs, whatever the exogenous BCAAs provided. The biosynthesis of short BCFAs is closely related to that of membrane BCFAs in P. freudenreichii. The biosynthesis of short BCFAs in P. freudenreichii depends more on the strain than on the presence of exogenous BC precursors.
ISSN:1364-5072
1365-2672
DOI:10.1111/j.1365-2672.2008.03830.x