Genetic linkage maps of rose constructed with new microsatellite markers and locating QTL controlling flowering traits

New microsatellites markers [simple sequence repeat (SSR)] have been isolated from rose and integrated into an existing amplified fragment-length polymorphism genetic map. This new map was used to identify quantitative trait locus (QTL) controlling date of flowering and number of petals. From a rose...

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Bibliographic Details
Published in:Tree genetics & genomes 2008-01, Vol.4 (1), p.11-23
Main Authors: Hibrand-Saint Oyant, L, Crespel, L, Rajapakse, S, Zhang, L, Foucher, F
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biotechnology
Flowering traits
Forestry
Gene mapping
Genetic map
Genetic markers
Genetics
Genomics
Life Sciences
Original Paper
Plant Breeding/Biotechnology
Plant Genetics and Genomics
Plants genetics
Rosa
Rosaceae
Tree Biology
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Summary:New microsatellites markers [simple sequence repeat (SSR)] have been isolated from rose and integrated into an existing amplified fragment-length polymorphism genetic map. This new map was used to identify quantitative trait locus (QTL) controlling date of flowering and number of petals. From a rose bud expressed sequence tag (EST) database of 2,556 unigenes and a rose genomic library, 44 EST-SSRs and 20 genomic-SSR markers were developed, respectively. These new rose SSRs were used to expand genetic maps of the rose interspecific F₁ progeny. In addition, SSRs from other Rosaceae genera were also tested in the mapping progeny. Genetic maps for the two parents of the progeny were constructed using pseudo-testcross mapping strategy. The maps consist of seven linkage groups of 105 markers covering 432 cM for the maternal map and 136 markers covering 438 cM for the paternal map. Homologous relationships among linkage groups between the maternal and paternal maps were established using SSR markers. Loci controlling flowering traits were localised on genetic maps as a major gene and QTL for the number of petals and a QTL for the blooming date. New SSR markers developed in this study will provide tools for the establishment of a consensus linkage map for roses that combine traits and markers in various rose genetic maps.
ISSN:1614-2942
1614-2950
DOI:10.1007/s11295-007-0084-2