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31P nuclear magnetic resonance measurements of phosphate metabolites and intracellular pH in turbot Psetta maxima red blood cells using a novel flow method
A method for oxygenating and mixing suspensions of turbot Psetta maxima red blood cells (RBC) was tested in (31)P nuclear magnetic resonance (NMR) spectroscopy. In normoxia, the levels of inorganic phosphate (Pi) and nucleoside triphosphates (NTP) were stable up to 140 min and intracellular pH (pHi)...
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| Published in: | Journal of fish biology 2009-08, Vol.75 (3), p.747-754 |
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| Format: | Article |
| Language: | English |
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| container_end_page | 754 |
| container_issue | 3 |
| container_start_page | 747 |
| container_title | Journal of fish biology |
| container_volume | 75 |
| creator | Thebault, M T Pichavant, K Kervarec, N |
| description | A method for oxygenating and mixing suspensions of turbot Psetta maxima red blood cells (RBC) was tested in (31)P nuclear magnetic resonance (NMR) spectroscopy. In normoxia, the levels of inorganic phosphate (Pi) and nucleoside triphosphates (NTP) were stable up to 140 min and intracellular pH (pHi) was maintained and decreased oxygen partial pressure (P(O ( 2) )) from 30 to 15 and 600 Pa produced a significant fall in the intensity of NTP resonance, balanced by an increase in the Pi signal. Treatment of RBC with 0. 5 M isoproterenol during hypoxia exposure did not affect the pattern of changes in NTP or pHi induced by hypoxia and the effect was manifest only on Pi levels. |
| doi_str_mv | 10.1111/j.1095-8649.2009.02351.x |
| format | article |
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In normoxia, the levels of inorganic phosphate (Pi) and nucleoside triphosphates (NTP) were stable up to 140 min and intracellular pH (pHi) was maintained and decreased oxygen partial pressure (P(O ( 2) )) from 30 to 15 and 600 Pa produced a significant fall in the intensity of NTP resonance, balanced by an increase in the Pi signal. Treatment of RBC with 0. 5 M isoproterenol during hypoxia exposure did not affect the pattern of changes in NTP or pHi induced by hypoxia and the effect was manifest only on Pi levels.</description><identifier>ISSN: 0022-1112</identifier><identifier>EISSN: 1095-8649</identifier><identifier>DOI: 10.1111/j.1095-8649.2009.02351.x</identifier><identifier>PMID: 20738573</identifier><language>eng</language><publisher>England: Wiley</publisher><subject>Animals ; Cell Respiration ; Cell Respiration - physiology ; Erythrocytes ; Erythrocytes - chemistry ; Erythrocytes - metabolism ; Flatfishes ; Flatfishes - metabolism ; Flatfishes - physiology ; Human health and pathology ; Hydrogen-Ion Concentration ; Intracellular Space ; Intracellular Space - chemistry ; Intracellular Space - metabolism ; Life Sciences ; Magnetic Resonance Spectroscopy ; Marine ; Monitoring, Physiologic ; Monitoring, Physiologic - instrumentation ; Monitoring, Physiologic - methods ; Phosphates ; Phosphates - analysis ; Phosphates - metabolism ; Phosphorus Isotopes ; Phosphorus Isotopes - analysis ; Scophthalmus maximus ; Tissues and Organs</subject><ispartof>Journal of fish biology, 2009-08, Vol.75 (3), p.747-754</ispartof><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20738573$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.univ-brest.fr/hal-00750436$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Thebault, M T</creatorcontrib><creatorcontrib>Pichavant, K</creatorcontrib><creatorcontrib>Kervarec, N</creatorcontrib><title>31P nuclear magnetic resonance measurements of phosphate metabolites and intracellular pH in turbot Psetta maxima red blood cells using a novel flow method</title><title>Journal of fish biology</title><addtitle>J Fish Biol</addtitle><description>A method for oxygenating and mixing suspensions of turbot Psetta maxima red blood cells (RBC) was tested in (31)P nuclear magnetic resonance (NMR) spectroscopy. In normoxia, the levels of inorganic phosphate (Pi) and nucleoside triphosphates (NTP) were stable up to 140 min and intracellular pH (pHi) was maintained and decreased oxygen partial pressure (P(O ( 2) )) from 30 to 15 and 600 Pa produced a significant fall in the intensity of NTP resonance, balanced by an increase in the Pi signal. Treatment of RBC with 0. 5 M isoproterenol during hypoxia exposure did not affect the pattern of changes in NTP or pHi induced by hypoxia and the effect was manifest only on Pi levels.</description><subject>Animals</subject><subject>Cell Respiration</subject><subject>Cell Respiration - physiology</subject><subject>Erythrocytes</subject><subject>Erythrocytes - chemistry</subject><subject>Erythrocytes - metabolism</subject><subject>Flatfishes</subject><subject>Flatfishes - metabolism</subject><subject>Flatfishes - physiology</subject><subject>Human health and pathology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Intracellular Space</subject><subject>Intracellular Space - chemistry</subject><subject>Intracellular Space - metabolism</subject><subject>Life Sciences</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Marine</subject><subject>Monitoring, Physiologic</subject><subject>Monitoring, Physiologic - instrumentation</subject><subject>Monitoring, Physiologic - methods</subject><subject>Phosphates</subject><subject>Phosphates - analysis</subject><subject>Phosphates - metabolism</subject><subject>Phosphorus Isotopes</subject><subject>Phosphorus Isotopes - analysis</subject><subject>Scophthalmus maximus</subject><subject>Tissues and Organs</subject><issn>0022-1112</issn><issn>1095-8649</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNp9kctu1TAQQC0EopfCLyCvQF0kjF-xs6wq4CJdiS5gHTnJpEnl2CF2SvkWfraOWrpkNiPNHB3NgxDKoGQ5Pt2WDGpVmErWJQeoS-BCsfL-BTk8N16SAwDnReb5GXkT4y1kUtTiNTnjoIVRWhzIX8Guqd86h3als73xmKaOrhiDt75DOqON24oz-hRpGOgyhriMNu2dZNvgpoSRWt_TyafVdujc5rJqOeYCTdvahkSvI6Zks_5-mm2W97R1IfR0pyPd4uRvqKU-3KGjgwu_d_cY-rfk1WBdxHdP-Zz8_PL5x9WxOH3_-u3q8lSMHFQqlEFTV4oZYJ1RwlYtVBVUmsnOalMLOaAWCFpK3Q6iYlUPPTdM1rKHVrdSnJOLR-9oXbOsecb1TxPs1BwvT81eA9AKpKjuWGY_PrLLGn5tGFMzT3Hfw3oMW2y0NHU-shSZ_PBfkjMwiiuVwfdP4NbO2D9P8O9J4gH7SJXo</recordid><startdate>200908</startdate><enddate>200908</enddate><creator>Thebault, M T</creator><creator>Pichavant, K</creator><creator>Kervarec, N</creator><general>Wiley</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QG</scope><scope>7SN</scope><scope>7TN</scope><scope>C1K</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope><scope>1XC</scope></search><sort><creationdate>200908</creationdate><title>31P nuclear magnetic resonance measurements of phosphate metabolites and intracellular pH in turbot Psetta maxima red blood cells using a novel flow method</title><author>Thebault, M T ; Pichavant, K ; Kervarec, N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h205t-58e89651801c853a6b06606714ca78934fe73e07447bf3616d0d281494d0b7b43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Cell Respiration</topic><topic>Cell Respiration - physiology</topic><topic>Erythrocytes</topic><topic>Erythrocytes - chemistry</topic><topic>Erythrocytes - metabolism</topic><topic>Flatfishes</topic><topic>Flatfishes - metabolism</topic><topic>Flatfishes - physiology</topic><topic>Human health and pathology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Intracellular Space</topic><topic>Intracellular Space - chemistry</topic><topic>Intracellular Space - metabolism</topic><topic>Life Sciences</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Marine</topic><topic>Monitoring, Physiologic</topic><topic>Monitoring, Physiologic - instrumentation</topic><topic>Monitoring, Physiologic - methods</topic><topic>Phosphates</topic><topic>Phosphates - analysis</topic><topic>Phosphates - metabolism</topic><topic>Phosphorus Isotopes</topic><topic>Phosphorus Isotopes - analysis</topic><topic>Scophthalmus maximus</topic><topic>Tissues and Organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thebault, M T</creatorcontrib><creatorcontrib>Pichavant, K</creatorcontrib><creatorcontrib>Kervarec, N</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Animal Behavior Abstracts</collection><collection>Ecology Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Journal of fish biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thebault, M T</au><au>Pichavant, K</au><au>Kervarec, N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>31P nuclear magnetic resonance measurements of phosphate metabolites and intracellular pH in turbot Psetta maxima red blood cells using a novel flow method</atitle><jtitle>Journal of fish biology</jtitle><addtitle>J Fish Biol</addtitle><date>2009-08</date><risdate>2009</risdate><volume>75</volume><issue>3</issue><spage>747</spage><epage>754</epage><pages>747-754</pages><issn>0022-1112</issn><eissn>1095-8649</eissn><abstract>A method for oxygenating and mixing suspensions of turbot Psetta maxima red blood cells (RBC) was tested in (31)P nuclear magnetic resonance (NMR) spectroscopy. In normoxia, the levels of inorganic phosphate (Pi) and nucleoside triphosphates (NTP) were stable up to 140 min and intracellular pH (pHi) was maintained and decreased oxygen partial pressure (P(O ( 2) )) from 30 to 15 and 600 Pa produced a significant fall in the intensity of NTP resonance, balanced by an increase in the Pi signal. Treatment of RBC with 0. 5 M isoproterenol during hypoxia exposure did not affect the pattern of changes in NTP or pHi induced by hypoxia and the effect was manifest only on Pi levels.</abstract><cop>England</cop><pub>Wiley</pub><pmid>20738573</pmid><doi>10.1111/j.1095-8649.2009.02351.x</doi><tpages>8</tpages></addata></record> |
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| ispartof | Journal of fish biology, 2009-08, Vol.75 (3), p.747-754 |
| issn | 0022-1112 1095-8649 |
| language | eng |
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| source | Wiley |
| subjects | Animals Cell Respiration Cell Respiration - physiology Erythrocytes Erythrocytes - chemistry Erythrocytes - metabolism Flatfishes Flatfishes - metabolism Flatfishes - physiology Human health and pathology Hydrogen-Ion Concentration Intracellular Space Intracellular Space - chemistry Intracellular Space - metabolism Life Sciences Magnetic Resonance Spectroscopy Marine Monitoring, Physiologic Monitoring, Physiologic - instrumentation Monitoring, Physiologic - methods Phosphates Phosphates - analysis Phosphates - metabolism Phosphorus Isotopes Phosphorus Isotopes - analysis Scophthalmus maximus Tissues and Organs |
| title | 31P nuclear magnetic resonance measurements of phosphate metabolites and intracellular pH in turbot Psetta maxima red blood cells using a novel flow method |
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