Application of solid-phase extraction to agar-supported fermentation

Agar-supported fermentation (Ag-SF), a variant of solid-state fermentation, has recently been improved by the development of a dedicated 2 m 2 scale pilot facility, Platotex. We investigated the application of solid-phase extraction (SPE) to Ag-SF in order to increase yields and minimize the contami...

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Bibliographic Details
Published in:Bioprocess and biosystems engineering 2013-09, Vol.36 (9), p.1285-1290
Main Authors: Le Goff, Géraldine, Adelin, Emilie, Cortial, Sylvie, Servy, Claudine, Ouazzani, Jamal
Format: Article
Language:English
Subjects:
Agar - chemistry
Biofilms
Biotechnology
Chemical Sciences
Chemistry
Chemistry and Materials Science
Culture Media - chemistry
Environmental Engineering/Biotechnology
Environmental impact
Extraction processes
Fermentation
Food Science
Fungi
Fusarium
Fusarium - growth & development
Industrial and Production Engineering
Industrial Chemistry/Chemical Engineering
Metabolites
Mycelium - growth & development
Organic chemistry
Original Paper
Phomopsis
Polystyrenes - chemistry
Resins
Secondary metabolites
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Summary:Agar-supported fermentation (Ag-SF), a variant of solid-state fermentation, has recently been improved by the development of a dedicated 2 m 2 scale pilot facility, Platotex. We investigated the application of solid-phase extraction (SPE) to Ag-SF in order to increase yields and minimize the contamination of the extracts with agar constituents. The selection of the appropriate resin was conducted on liquid-state fermentation and Diaion HP-20 exhibited the highest recovery yield and selectivity for the metabolites of the model fungal strains Phomopsis sp. and Fusarium sp. SPE applied to Ag-SF resulted in a particular compartmentalization of the culture. The mycelium that requires oxygen to grow migrates to the top layer and formed a thick biofilm. The resin beads intercalate between the agar surface and the mycelium layer, and trap directly the compounds secreted by the mycelium through a “solid–solid extraction” (SSE) process. The resin/mycelium layer is easily recovered by scraping the surface and the target metabolites extracted by methanol. Ag-SF associated to SSE represents an ideal compromise for the production of bioactive secondary metabolites with limited economic and environmental impact.
ISSN:1615-7591
1615-7605
DOI:10.1007/s00449-012-0873-3