Phylogenetic character mapping of proteomic diversity shows high correlation with subspecific phylogenetic diversity in Trypanosoma cruzi

We performed a phylogenetic character mapping on 26 stocks of Trypanosoma cruzi, the parasite responsible for Chagas disease, and 2 stocks of the sister taxon T. cruzi marinkellei to test for possible associations between T. cruzi—subspecific phylogenetic diversity and levels of protein expression,...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2010-11, Vol.107 (47), p.20411-20416
Main Authors: Telleria, Jenny, Biron, David G., Brizard, Jean-Paul, Demettre, Edith, Séveno, Martial, Barnabé, Christian, Ayala, Francisco J., Tibayrenc, Michel
Format: Article
Language:English
Subjects:
Biodiversity
Biodiversity and Ecology
Biological Sciences
Biomarkers
Chagas disease
Cluster Analysis
Electrophoresis
Electrophoresis, Gel, Two-Dimensional
Environmental Sciences
Evolution
Evolutionary genetics
Gels
Gene Expression
Genetic diversity
Mass Spectrometry
Parasites
Parasitic diseases
Parasitism
Pathogenesis
Phylogenetics
Phylogeny
Proteins
Proteomics
Proteomics - methods
Protozoan Proteins - genetics
Protozoan Proteins - metabolism
Species Specificity
Trypanosoma cruzi
Trypanosoma cruzi - genetics
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Summary:We performed a phylogenetic character mapping on 26 stocks of Trypanosoma cruzi, the parasite responsible for Chagas disease, and 2 stocks of the sister taxon T. cruzi marinkellei to test for possible associations between T. cruzi—subspecific phylogenetic diversity and levels of protein expression, as examined by proteomic analysis and mass spectrometry. We observed a high level of correlation (P < 10⁻⁴) between genetic distance, as established by multilocus enzyme electrophoresis, and proteomic dissimilarities estimated by proteomic Euclidian distances. Several proteins were found to be specifically associated to T. cruzi phylogenetic subdivisions (discrete typing units). This study explores the previously uncharacterized links between infraspecific phylogenetic diversity and gene expression in a human pathogen. It opens the way to searching for new vaccine and drug targets and for identification of specific biomarkers at the subspecific level of pathogens.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.1015496107