Development of a field-portable fluorometer based on deep ultraviolet LEDs for the detection of phenanthrene- and tryptophan-like compounds in natural waters

In recent years, portable or submersible fluorometers have become key tools in acquiring real time high frequency measurements of target dissolved organic matter (DOM) fluorophores in aquatic environments. We have developed an original field-portable fluorometer based on deep UV light-emitting diode...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2013, Vol.182, p.416-423
Main Authors: Tedetti, Marc, Joffre, Pascal, Goutx, Madeleine
Format: Article
Language:English
Subjects:
aquatic environment
coastal water
coasts
detection limit
Dissolved organic matter
effluents
Environment and Society
Environmental Sciences
Field fluorometer
Fluorescence
Global Changes
Ocean, Atmosphere
Phenanthrene
Sciences of the Universe
sewage
Tryptophan
UV LED
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Summary:In recent years, portable or submersible fluorometers have become key tools in acquiring real time high frequency measurements of target dissolved organic matter (DOM) fluorophores in aquatic environments. We have developed an original field-portable fluorometer based on deep UV light-emitting diodes (LEDs) for simultaneous measurements of two DOM fluorophores in natural waters: phenanthrene- and tryptophan-like compounds. This fluorometer, called “the MiniFluo-UV” was tested on standard solutions and natural samples collected on the northwestern Mediterranean coast (vicinity of Marseille City, France). The MiniFluo-UV responded linearly to an increase in phenanthrene and tryptophan concentrations over the range 0.1–100μgl−1. Limits of detection for these two fluorophores were 1.43 and 0.72μgl−1, respectively. The sensor also responded linearly to an increase in measurement duration over the range 200–2000ms, and the fluorescence intensity showed a power decrease from calibre C0 (integration capacitor of 3pF) to calibre C3 (integration capacitor of 73pF). Optimal parameters (duration of 600ms, calibre C0) were used for measurements in natural water samples. We found very significant linear relationships between the phenanthrene and tryptophan equivalent concentrations measured with the MiniFluo-UV and the concentrations measured with the Hitachi laboratory spectrofluorometer on all natural water samples (R2=0.90 and 0.98, n=150, p
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2013.03.052