Influence of surface energy distribution on neuritogenesis

PC12 cells are a useful model to study neuronal differentiation, as they can undergo terminal differentiation, typically when treated with nerve growth factor (NGF). In this study we investigated the influence of surface energy distribution on PC12 cell differentiation, by atomic force microscopy (A...

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Published in:Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2009-09, Vol.72 (2), p.208-218
Main Authors: Lamour, Guillaume, Journiac, Nathalie, Souès, Sylvie, Bonneau, Stéphanie, Nassoy, Pierre, Hamraoui, Ahmed
Format: Article
Language:English
Subjects:
Animals
Atomic force microscopy
Biological Physics
Cell Differentiation - drug effects
Cell Differentiation - physiology
Fluorescent Antibody Technique
Glass - chemistry
Microscopy, Atomic Force
Nanostructures - chemistry
Nerve Growth Factor - pharmacology
Neurite outgrowth
Neurites
Neurogenesis - drug effects
Neurogenesis - physiology
PC12 Cells
Physics
Rats
Self-assembled monolayers
Silanes - chemistry
Surface chemistry
Surface energy
Surface Properties
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Summary:PC12 cells are a useful model to study neuronal differentiation, as they can undergo terminal differentiation, typically when treated with nerve growth factor (NGF). In this study we investigated the influence of surface energy distribution on PC12 cell differentiation, by atomic force microscopy (AFM) and immunofluorescence. Glass surfaces were modified by chemisorption: an aminosilane, n-[3-(trimethoxysilyl)propyl]ethylendiamine (C 8H 22N 2O 3Si; EDA), was grafted by polycondensation. AFM analysis of substrate topography showed the presence of aggregates suggesting that the adsorption is heterogeneous, and generates local gradients in energy of adhesion. PC12 cells cultured on these modified glass surfaces developed neurites in absence of NGF treatment. In contrast, PC12 cells did not grow neurites when cultured in the absence of NGF on a relatively smooth surface such as poly- l-lysine substrate, where amine distribution is rather homogeneous. These results suggest that surface energy distribution, through cell–substrate interactions, triggers mechanisms that will drive PC12 cells to differentiate and to initiate neuritogenesis. We were able to create a controlled physical nano-structuration with local variations in surface energy that allowed the study of these parameters on neuritogenesis.
ISSN:0927-7765
1873-4367
DOI:10.1016/j.colsurfb.2009.04.006