Subpicosecond UV spectroscopy of carbonmonoxy-myoglobin : absorption and circular dichroism studies

A thorough absorption and circular dichroism study is performed in carbonmonoxy-myoglobin with a sub-picosecond visible pump, ultraviolet probe experiment. Differential absorption in the 220-360 nm range shows that the time-resolved response mainly comes from the heme and that aromatic amino acids d...

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Bibliographic Details
Published in:Physical chemistry chemical physics : PCCP 2007-01, Vol.9 (13), p.1611-1615
Main Authors: DARTIGALONGUE, Thibault, NIEZBORALA, Claire, HACHE, Francois
Format: Article
Language:English
Subjects:
Absorption
Chemistry
Circular Dichroism - methods
Exact sciences and technology
General and physical chemistry
Myoglobin - chemistry
Spectrophotometry, Ultraviolet - methods
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Summary:A thorough absorption and circular dichroism study is performed in carbonmonoxy-myoglobin with a sub-picosecond visible pump, ultraviolet probe experiment. Differential absorption in the 220-360 nm range shows that the time-resolved response mainly comes from the heme and that aromatic amino acids do not contribute significantly. Time-resolved CD at 260 nm shows no dynamics and confirms this result. On the contrary, a strong CD dynamics is observed at 230 nm. This signal could originate from transient deformation of the alpha-helices in the protein.
ISSN:1463-9076
1463-9084
DOI:10.1039/b616173a