Nanocarriers with ultrahigh chromophore loading for fluorescence bio-imaging and photodynamic therapy

Abstract We describe the design of original nanocarriers that allows for ultrahigh chromophore loading while maintaining the photo-activity of each individual molecule. They consist in shells of charged biocompatible polymers grafted on gold nanospheres. The self-organization of extended polymer cha...

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Bibliographic Details
Published in:Biomaterials 2013-11, Vol.34 (33), p.8344-8351
Main Authors: Navarro, Julien R.G, Lerouge, Frédéric, Cepraga, Cristina, Micouin, Guillaume, Favier, Arnaud, Chateau, Denis, Charreyre, Marie-Thérèse, Lanoë, Pierre-Henri, Monnereau, Cyrille, Chaput, Frédéric, Marotte, Sophie, Leverrier, Yann, Marvel, Jacqueline, Kamada, Kenji, Andraud, Chantal, Baldeck, Patrice L, Parola, Stephane
Format: Article
Language:English
Subjects:
Advanced Basic Science
Bio-imaging
Cell Line, Tumor
Chemical Sciences
Dentistry
Fluorescence
Gold
Gold - chemistry
Humans
Material chemistry
Molecular Structure
Nanocarriers
Nanoparticles - chemistry
Optical Imaging - methods
PDT
Photochemotherapy - methods
Polymers
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Summary:Abstract We describe the design of original nanocarriers that allows for ultrahigh chromophore loading while maintaining the photo-activity of each individual molecule. They consist in shells of charged biocompatible polymers grafted on gold nanospheres. The self-organization of extended polymer chains results from repulsive charges and steric interactions that are optimized by tuning the surface curvature of nanoparticles. This type of nano-scaffolds can be used as light-activated theranostic agents for fluorescence imaging and photodynamic therapy. We demonstrate that, labeled with a fluorescent photosensitizer, it can localize therapeutic molecules before triggering the cell death of B16-F10 melanoma with an efficiency that is similar to the efficiency of the polymer conjugate alone, and with the advantage of extremely high local loading of photosensitizers (object concentration in the picomolar range).
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2013.07.032