Multi-scale engineering for neuronal cell growth and differentiation

In this paper we investigate the role of micropatterning and molecular coating for cell culture and differentiation of neuronal cells (Neuro2a cell line) on a polydimethylsiloxane substrate. We investigate arrays of micrometric grooves (line and space) capable to guide neurite along their axis. We d...

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Bibliographic Details
Published in:Microelectronic engineering 2011-08, Vol.88 (8), p.1668-1671
Main Authors: Béduer, Amélie, Vaysse, Laurence, Flahaut, Emmanuel, Seichepine, Florent, Loubinoux, Isabelle, Vieu, Christophe
Format: Article
Language:English
Subjects:
Applied sciences
Arrays
Carbon nanotubes
Cell patterning
Coating
Cross-disciplinary physics: materials science
rheology
Culture
Density
Differentiation
Engineering Sciences
Exact sciences and technology
Grooves
Life Sciences
Materials science
Metals. Metallurgy
Methods of nanofabrication
Micro and nanotechnologies
Microelectronics
Micropatterning
Microtopography
Nanoscale materials and structures: fabrication and characterization
Nanoscale pattern formation
Nanotopography
Nanotubes
Neuronal cells
Neurons and Cognition
Optimization
Physics
Production techniques
Surface treatment
Surface treatments
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Summary:In this paper we investigate the role of micropatterning and molecular coating for cell culture and differentiation of neuronal cells (Neuro2a cell line) on a polydimethylsiloxane substrate. We investigate arrays of micrometric grooves (line and space) capable to guide neurite along their axis. We demonstrate that pattern dimensions play a major role due to the deformation of the cell occasioned by grooves narrower than typical cell dimension. A technological compromise for optimizing cell density, differentiation rate and neurite alignment has been obtained for 20μm wide grooves which is a dimension comparable with the average cell dimension. This topographical engineered pattern combined with double-wall carbon nanotubes coating enabled us to obtain adherent cell densities in the order of 104cells/cm2 and a differentiation rate close to 100%.
ISSN:0167-9317
1873-5568
DOI:10.1016/j.mee.2010.12.049