Norovirus Capsid Proteins Self-Assemble through Biphasic Kinetics via Long-Lived Stave-like Intermediates

• Published in: Journal of the American Chemical Society 2013-10, Vol.135 (41), p.15373-15381
• Main Authors: Tresset, Guillaume, Le Coeur, Clémence, Bryche, Jean-François, Tatou, Mouna, Zeghal, Mehdi, Charpilienne, Annie, Poncet, Didier, Constantin, Doru, Bressanelli, Stéphane
• Format: Article
• Language: English
• Subjects: Capsid Proteins - chemistry; Capsid Proteins - isolation & purification; Capsid Proteins - metabolism; Condensed Matter; Kinetics; Norovirus - chemistry; Physics; Quantum Theory; Scattering, Small Angle; Soft Condensed Matter; Time Factors; X-Ray Diffraction
• ISSN: 0002-7863; 1520-5126
• DOI: 10.1021/ja403550f

The self-assembly kinetics for a norovirus capsid protein were probed by time-resolved small-angle X-ray scattering and then analyzed by singular value decomposition and global fitting. Only three species contribute to the total scattering intensities: dimers, intermediates comprising some 11 dimers, and icosahedral T = 3 capsids made up of 90 dimers. Three-dimensional reconstructions of the intermediate robustly show a stave-like shape consistent with an arrangement of two pentameric units connected by an interstitial dimer. Upon triggering of self-assembly, the biphasic kinetics consist of a fast step in which dimers are assembled into intermediates, followed by a slow step in which intermediates interlock into capsids. This simple kinetic model reproduces experimental data with an excellent agreement over 6 decades in time and with nanometer resolution. The extracted form factors are robust against changes in experimental conditions. These findings challenge and complement currently accepted models for the assembly of norovirus capsids.