Interaction of Antibiotics with Lipid Vesicles on Thin Film Porous Silicon Using Reflectance Interferometric Fourier Transform Spectroscopy

The ability to observe interactions of drugs with cell membranes is an important area in pharmaceutical research. However, these processes are often difficult to understand due to the dynamic nature of cell membranes. Therefore, artificial systems composed of lipids have been used to study membrane...

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Bibliographic Details
Published in:Langmuir 2013-08, Vol.29 (32), p.10279-10286
Main Authors: Guinan, Taryn, Godefroy, Cédric, Lautrédou, Nicole, Pace, Stephanie, Milhiet, Pierre-Emmanuel, Voelcker, Nicolas, Cunin, Frédérique
Format: Article
Language:English
Subjects:
Adsorption
Anti-Bacterial Agents - chemistry
Biotechnology
Chemical Sciences
Chemistry
Colloidal state and disperse state
Computer Science
Exact sciences and technology
General and physical chemistry
Life Sciences
Lipids - chemistry
Material chemistry
Membranes
Particle Size
Porosity
Porous materials
Silicon - chemistry
Spectroscopy, Fourier Transform Infrared
Surface Properties
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Summary:The ability to observe interactions of drugs with cell membranes is an important area in pharmaceutical research. However, these processes are often difficult to understand due to the dynamic nature of cell membranes. Therefore, artificial systems composed of lipids have been used to study membrane properties and their interaction with drugs. Here, lipid vesicle adsorption, rupture, and formation of planar lipid bilayers induced by various antibiotics (surfactin, azithromycin, gramicidin, melittin and ciprofloxacin) and the detergent dodecyl-b-d-thiomaltoside (DOTM) was studied using reflective interferometric Fourier transform spectroscopy (RIFTS) on an oxidized porous silicon (pSi) surface as a transducer. The pSi transducer surfaces are prepared as thin films of 3 μm thickness with pore dimensions of a few nanometers in diameter by electrochemical etching of crystalline silicon followed by passivation with a thermal oxide layer. Furthermore, the sensitivity of RIFTS was investigated using three different concentrations of surfactin. Complementary techniques including atomic force microscopy, fluorescence recovery after photobleaching, and fluorescence microscopy were used to validate the RIFTS-based method and confirm adsorption and consequent rupture of vesicles to form a phospholipid bilayer upon the addition of antibiotics. The method provides a sensitive and real-time approach to monitor the antibiotic-induced transition of lipid vesicles to phospholipid bilayers.
ISSN:0743-7463
1520-5827
DOI:10.1021/la401804e