Paramagnetic nanoparticles to track and quantify in vivo immune human therapeutic cells

This study aims to investigate gadolinium-based nanoparticles (Gd-HNP) for in vitro labeling of human plasmacytoid dendritic cells (HuPDC) to allow for in vivo tracking and HuPDC quantifying using magnetic resonance imaging (MRI) following parenteral injection. Human plasmacytoid DC were labeled (La...

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Bibliographic Details
Published in:Nanoscale 2013-12, Vol.5 (23), p.11409-11415
Main Authors: Aspord, Caroline, Laurin, David, Janier, Marc F, Mandon, Céline A, Thivolet, Charles, Villiers, Christian, Mowat, Pierre, Madec, Anne-Marie, Tillement, Olivier, Perriat, Pascal, Louis, Cédric, Bérard, Frédéric, Marche, Patrice N, Plumas, Joël, Billotey, Claire
Format: Article
Language:English
Subjects:
Animals
Biochemistry, Molecular Biology
Cell Line
Cell Tracking
Contrast Media - chemical synthesis
Contrast Media - chemistry
Contrast Media - pharmacokinetics
Dendritic Cells - chemistry
Dendritic Cells - cytology
Dendritic Cells - transplantation
Fluorescein-5-isothiocyanate - chemistry
Gadolinium - chemistry
Humans
Injections, Intraperitoneal
Injections, Intravenous
Life Sciences
Magnetic Resonance Imaging
Magnetite Nanoparticles - chemistry
Mice
Mice, Inbred NOD
Mice, SCID
Spleen - immunology
Spleen - metabolism
Tissue Distribution
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Summary:This study aims to investigate gadolinium-based nanoparticles (Gd-HNP) for in vitro labeling of human plasmacytoid dendritic cells (HuPDC) to allow for in vivo tracking and HuPDC quantifying using magnetic resonance imaging (MRI) following parenteral injection. Human plasmacytoid DC were labeled (LabHuPDC) with fluorescent Gd-HNP (Gd-FITC-HNP) and injected via intraperitoneal and intravenous routes in 4-5 NOD-SCID β2m(-/-)mice (treated mice = TM). Control mice (CM) were similarly injected with unlabeled HuPDC. In vivo 7 T MRI was performed 24 h later and all spleens were removed in order to measure Gd and fluorescence contents and identify HuPDC. Gd-FITC-HNP efficiently labeled HuPDC (0.05 to 0.1 pg per cell), without altering viability and activation properties. The magnetic resonance (MR) signal was exclusively due to HuPDC. The normalized MR splenic intensity for TM was significantly higher than for CM (p < 0.024), and highly correlated with the spleen Gd content (r = 0.97), and the number of HuPDC found in the spleen (r = 0.94). Gd-FITC-HNP allowed for in vivo tracking and HuPDC quantifying by means of MRI following parenteral injection, with very high sensitivity (
ISSN:2040-3364
2040-3372
DOI:10.1039/c3nr34240a