Fluorescence detection to determine proteins and humic-like substances fingerprints of exopolymeric substances (EPS) from biological sludges performed by size exclusion chromatography (SEC)

SEC chromatograms of EPS from anaerobic granular sludge (GS-Sm) with fluorescence (221nm–350nm) of protein-like molecules and fluorescence (345nm–443nm) of humic-like substances using a mobile phase (phosphate buffer (50mM) with NaCl (150mM) at pH 7.0±0.1) at flow-rate 0.5mL.min−1 with HMW Superdex...

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Bibliographic Details
Published in:Bioresource technology 2013-03, Vol.131, p.159-165
Main Authors: Bhatia, Divya, Bourven, Isabelle, Simon, Stéphane, Bordas, François, van Hullebusch, Eric D., Rossano, Stéphanie, Lens, Piet N.L., Guibaud, Gilles
Format: Article
Language:English
Subjects:
Analytical chemistry
Applied sciences
Bacteria
Bacteria, Anaerobic - metabolism
Bacterial Proteins - analysis
Bacterial Proteins - chemistry
Biological and medical sciences
Chemical compounds
Chemical Sciences
Chromatography, Gel - methods
Displays
Environmental Sciences
EPS
Exact sciences and technology
Extracellular polymeric substances (EPS)
Fingerprints
Fluorescence
Fluorescence detector
Fundamental and applied biological sciences. Psychology
Humic Substances - analysis
Humic Substances - microbiology
Humic-like substances
Pollution
Polysaccharides, Bacterial - chemistry
Protein-like macromolecules
Proteins
Sewage - microbiology
Size exclusion chromatography
Size exclusion chromatography (SEC)
Spectrometry, Fluorescence - methods
Wastes
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Summary:SEC chromatograms of EPS from anaerobic granular sludge (GS-Sm) with fluorescence (221nm–350nm) of protein-like molecules and fluorescence (345nm–443nm) of humic-like substances using a mobile phase (phosphate buffer (50mM) with NaCl (150mM) at pH 7.0±0.1) at flow-rate 0.5mL.min−1 with HMW Superdex 200 10/300 GL column, Amersham Biosciences. MWp: apparent molecular weight upon protein standard. [Display omitted] ► EPS were studied by size exclusion chromatography with UV and fluorescence detection. ► Fluorescence detection allows to obtain specific proteins and humic-like substances fingerprints. ► Changes in peak intensity and number are highlighted between UV and fluorescence detection. ► EPS display a protein-like fraction with a wide range of aMW (from >600kDa to
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2012.12.078