Fluorescence detection to determine proteins and humic-like substances fingerprints of exopolymeric substances (EPS) from biological sludges performed by size exclusion chromatography (SEC)

SEC chromatograms of EPS from anaerobic granular sludge (GS-Sm) with fluorescence (221nm–350nm) of protein-like molecules and fluorescence (345nm–443nm) of humic-like substances using a mobile phase (phosphate buffer (50mM) with NaCl (150mM) at pH 7.0±0.1) at flow-rate 0.5mL.min−1 with HMW Superdex...

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Published in:Bioresource technology 2013-03, Vol.131, p.159-165
Main Authors: Bhatia, Divya, Bourven, Isabelle, Simon, Stéphane, Bordas, François, van Hullebusch, Eric D., Rossano, Stéphanie, Lens, Piet N.L., Guibaud, Gilles
Format: Article
Language:English
Subjects:
Analytical chemistry
Applied sciences
Bacteria
Bacteria, Anaerobic - metabolism
Bacterial Proteins - analysis
Bacterial Proteins - chemistry
Biological and medical sciences
Chemical compounds
Chemical Sciences
Chromatography, Gel - methods
Displays
Environmental Sciences
EPS
Exact sciences and technology
Extracellular polymeric substances (EPS)
Fingerprints
Fluorescence
Fluorescence detector
Fundamental and applied biological sciences. Psychology
Humic Substances - analysis
Humic Substances - microbiology
Humic-like substances
Pollution
Polysaccharides, Bacterial - chemistry
Protein-like macromolecules
Proteins
Sewage - microbiology
Size exclusion chromatography
Size exclusion chromatography (SEC)
Spectrometry, Fluorescence - methods
Wastes
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cited_by cdi_FETCH-LOGICAL-c564t-5d0cc475bf36e4b12f55f4b52265c6ab49df6bb7b493809b41276b82a3b6a9133
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container_title Bioresource technology
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creator Bhatia, Divya
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Lens, Piet N.L.
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description SEC chromatograms of EPS from anaerobic granular sludge (GS-Sm) with fluorescence (221nm–350nm) of protein-like molecules and fluorescence (345nm–443nm) of humic-like substances using a mobile phase (phosphate buffer (50mM) with NaCl (150mM) at pH 7.0±0.1) at flow-rate 0.5mL.min−1 with HMW Superdex 200 10/300 GL column, Amersham Biosciences. MWp: apparent molecular weight upon protein standard. [Display omitted] ► EPS were studied by size exclusion chromatography with UV and fluorescence detection. ► Fluorescence detection allows to obtain specific proteins and humic-like substances fingerprints. ► Changes in peak intensity and number are highlighted between UV and fluorescence detection. ► EPS display a protein-like fraction with a wide range of aMW (from >600kDa to
doi_str_mv 10.1016/j.biortech.2012.12.078
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MWp: apparent molecular weight upon protein standard. [Display omitted] ► EPS were studied by size exclusion chromatography with UV and fluorescence detection. ► Fluorescence detection allows to obtain specific proteins and humic-like substances fingerprints. ► Changes in peak intensity and number are highlighted between UV and fluorescence detection. ► EPS display a protein-like fraction with a wide range of aMW (from &gt;600kDa to &lt;10kDa). ► EPS show a humic-like fraction with low aMW (from 6kDa to &lt;1.2kDa). Fingerprints of extracellular polymeric substances (EPS) from activated and anaerobic granular sludges were obtained by size exclusion chromatography coupled to UV (210 and 280nm) and fluorescence (221/350nm (protein-like molecules) and 345/443nm (humic-like substances)) detection. The total area below the peaks obtained with fluorescence detection is linked to the protein or humic-like substances EPS content. The EPS protein fingerprints, usually recorded with UV-280nm, change dramatically, mainly in the relative size of peaks when they were measured by a florescence detection method. It means that the apparent molecular weight (aMW) distribution of EPS chomatophores and fluorophores is different. Protein-like and humic-like substances were found to be specific fingerprints of the EPS, affected by the type and origin of the bacterial aggregate and improve EPS sample differentiation. 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Psychology ; Humic Substances - analysis ; Humic Substances - microbiology ; Humic-like substances ; Pollution ; Polysaccharides, Bacterial - chemistry ; Protein-like macromolecules ; Proteins ; Sewage - microbiology ; Size exclusion chromatography ; Size exclusion chromatography (SEC) ; Spectrometry, Fluorescence - methods ; Wastes</subject><ispartof>Bioresource technology, 2013-03, Vol.131, p.159-165</ispartof><rights>2012 Elsevier Ltd</rights><rights>2014 INIST-CNRS</rights><rights>Copyright © 2012 Elsevier Ltd. 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MWp: apparent molecular weight upon protein standard. [Display omitted] ► EPS were studied by size exclusion chromatography with UV and fluorescence detection. ► Fluorescence detection allows to obtain specific proteins and humic-like substances fingerprints. ► Changes in peak intensity and number are highlighted between UV and fluorescence detection. ► EPS display a protein-like fraction with a wide range of aMW (from &gt;600kDa to &lt;10kDa). ► EPS show a humic-like fraction with low aMW (from 6kDa to &lt;1.2kDa). Fingerprints of extracellular polymeric substances (EPS) from activated and anaerobic granular sludges were obtained by size exclusion chromatography coupled to UV (210 and 280nm) and fluorescence (221/350nm (protein-like molecules) and 345/443nm (humic-like substances)) detection. The total area below the peaks obtained with fluorescence detection is linked to the protein or humic-like substances EPS content. 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MWp: apparent molecular weight upon protein standard. [Display omitted] ► EPS were studied by size exclusion chromatography with UV and fluorescence detection. ► Fluorescence detection allows to obtain specific proteins and humic-like substances fingerprints. ► Changes in peak intensity and number are highlighted between UV and fluorescence detection. ► EPS display a protein-like fraction with a wide range of aMW (from &gt;600kDa to &lt;10kDa). ► EPS show a humic-like fraction with low aMW (from 6kDa to &lt;1.2kDa). Fingerprints of extracellular polymeric substances (EPS) from activated and anaerobic granular sludges were obtained by size exclusion chromatography coupled to UV (210 and 280nm) and fluorescence (221/350nm (protein-like molecules) and 345/443nm (humic-like substances)) detection. The total area below the peaks obtained with fluorescence detection is linked to the protein or humic-like substances EPS content. 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identifier ISSN: 0960-8524
ispartof Bioresource technology, 2013-03, Vol.131, p.159-165
issn 0960-8524
1873-2976
language eng
recordid cdi_hal_primary_oai_HAL_hal_00925209v1
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subjects Analytical chemistry
Applied sciences
Bacteria
Bacteria, Anaerobic - metabolism
Bacterial Proteins - analysis
Bacterial Proteins - chemistry
Biological and medical sciences
Chemical compounds
Chemical Sciences
Chromatography, Gel - methods
Displays
Environmental Sciences
EPS
Exact sciences and technology
Extracellular polymeric substances (EPS)
Fingerprints
Fluorescence
Fluorescence detector
Fundamental and applied biological sciences. Psychology
Humic Substances - analysis
Humic Substances - microbiology
Humic-like substances
Pollution
Polysaccharides, Bacterial - chemistry
Protein-like macromolecules
Proteins
Sewage - microbiology
Size exclusion chromatography
Size exclusion chromatography (SEC)
Spectrometry, Fluorescence - methods
Wastes
title Fluorescence detection to determine proteins and humic-like substances fingerprints of exopolymeric substances (EPS) from biological sludges performed by size exclusion chromatography (SEC)
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