Evaluation of the usefulness of six commercial agglutination assays for serologic diagnosis of toxoplasmosis

Abstract Six agglutination tests for detecting Toxoplasma gondii –specific antibodies (immunoglobulin G or M) in serum were performed and compared. In total, 599 sera were examined using direct and indirect agglutination assays. Sensitivity varied from 93.7% to 100% and specificity from 97.1% to 99....

Full description

Saved in:
Bibliographic Details
Published in:Diagnostic microbiology and infectious disease 2012-07, Vol.73 (3), p.231-235
Main Authors: Villard, Odile, Cimon, Bernard, Franck, Jacqueline, Fricker-Hidalgo, Hélène, Godineau, Nadine, Houze, Sandrine, Paris, Luc, Pelloux, Hervé, Villena, Isabelle, Candolfi, Ermanno
Format: Article
Language:English
Subjects:
Agglutination Tests
Agglutination Tests - methods
Antibodies, Protozoan
Antibodies, Protozoan - blood
Biological and medical sciences
Diagnostic Errors
Diagnostic Errors - statistics & numerical data
Fundamental and applied biological sciences. Psychology
Human protozoal diseases
Humans
Immunoglobulin G
Immunoglobulin G - blood
Immunoglobulin M
Immunoglobulin M - blood
Infectious Disease
Infectious diseases
Internal Medicine
Life Sciences
Medical sciences
Microbiology
Parasitic diseases
Protozoal diseases
Santé publique et épidémiologie
Sensitivity and Specificity
Serologic diagnosis
Serologic Tests
Serologic Tests - methods
Toxoplasma
Toxoplasma - immunology
Toxoplasma gondii
Toxoplasmosis
Toxoplasmosis - diagnosis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Six agglutination tests for detecting Toxoplasma gondii –specific antibodies (immunoglobulin G or M) in serum were performed and compared. In total, 599 sera were examined using direct and indirect agglutination assays. Sensitivity varied from 93.7% to 100% and specificity from 97.1% to 99.2%. In a selected population with interfering diseases, the percentage of false positives ranged from 4.3% to 10.9%. Although an overall agreement of 100% was found for chronic toxoplasmosis, sensitivity for the detection of confirmed acute toxoplasmosis ranged from 86.4% to 97.3%. Regarding the large variability in terms of the performance of the 6 assays, tests based on the hemagglutination principle were found to be better than the other agglutination tests for all the panels evaluated, meaning that they could be used as qualitative or semiquantitative low-cost screening assays.
ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2012.03.014