Profiling an electrospray plume by laser-induced fluorescence and Fraunhofer diffraction combined to mass spectrometry: influence of size and composition of droplets on charge-state distributions of electrosprayed proteins

We investigated how physico-chemical properties of charged droplets are affected by the electrospray process, using simultaneous in situ measurements by laser-induced fluorescence (LIF), Fraunhofer diffraction and mass spectrometry. For this purpose, we implemented a laser-induced-fluorescence profi...

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Bibliographic Details
Published in:Physical chemistry chemical physics : PCCP 2012-07, Vol.14 (26), p.9389-9396
Main Authors: GIROD, Marion, DAGANY, Xavier, STAFFORD, George, BOUTOU, Véronique, BROYER, Michel, ANTOINE, Rodolphe, DUGOURD, Philippe, MORDEHAI, Alex, LOVE, Craig, WERLICH, Mark, FJELDSTED, John
Format: Article
Language:English
Subjects:
Chemical Sciences
Chemistry
Cytochromes c - chemistry
Diffraction
Droplets
Evaporation
Exact sciences and technology
Fluorescence
Fluorescent Dyes - chemistry
General and physical chemistry
Lasers
Mass Spectrometry
Methanol - chemistry
Methyl alcohol
Particle Size
Plumes
Profiling
Proteins - chemistry
Solvents - chemistry
Spectrometry, Mass, Electrospray Ionization
Volatile Organic Compounds - chemistry
Water - chemistry
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Summary:We investigated how physico-chemical properties of charged droplets are affected by the electrospray process, using simultaneous in situ measurements by laser-induced fluorescence (LIF), Fraunhofer diffraction and mass spectrometry. For this purpose, we implemented a laser-induced-fluorescence profiling setup in conjunction with a fast, high-resolution particle sizing scheme on a modified Agilent Jet Stream electrospray source coupled to a single quadrupole mass analyser. The optical setup permits us to profile the solvent fractionation and the size of the droplets as they evaporate in an electrospray plume by measuring both the angular scattering pattern and emission spectra of a solvatochromic fluorescent dye. Mass spectra are recorded simultaneously. These mass spectrometry and optical spectroscopy investigations allow us to study the relation between the observed charge-state distributions of protein anions and physico-chemical properties of evaporating droplets in the spray plume. By mixing water with methanol, a refolding of cytochrome C is observed as the water percentage increases in the plume due to the preponderant evaporation of volatile methanol.
ISSN:1463-9076
1463-9084
DOI:10.1039/c2cp23341j