Discriminating bacteria from other atmospheric particles using femtosecond molecular dynamics

We investigated femtosecond pump–repump depletion schemes in biological fluorophors (tryptophan and riboflavin) in order to discriminate bioaerosols from organic interferents emitted by combustion (traffic related urban aerosols). Although fluorescence depletion is significative for riboflavin (Rbf,...

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Bibliographic Details
Published in:Journal of photochemistry and photobiology. A, Chemistry. Chemistry., 2006-06, Vol.180 (3), p.300-306
Main Authors: Courvoisier, François, Boutou, Véronique, Guyon, Laurent, Roth, Matthias, Rabitz, Herschel, Wolf, Jean-Pierre
Format: Article
Language:English
Subjects:
Aerosol particles discrimination
Bio-molecules ultrafast spectroscopy
Biological intrinsic fluorophore
Femtosecond fluorescence depletion
Optics
Physics
Real-time particle detection
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Summary:We investigated femtosecond pump–repump depletion schemes in biological fluorophors (tryptophan and riboflavin) in order to discriminate bioaerosols from organic interferents emitted by combustion (traffic related urban aerosols). Although fluorescence depletion is significative for riboflavin (Rbf, Vitamin B2), the most striking results have been obtained for the amino acid tryptophan (Trp). By using a 270 nm-pump 810 nm-repump femtosecond excitation, we showed that Trp exhibits fluorescence depletion up to 50%, contrary to naphthalene (
ISSN:1010-6030
1873-2666
DOI:10.1016/j.jphotochem.2006.03.039