Proteomic analysis of peripheral layers during wheat (Triticum aestivum L.) grain development

Grains of hexaploid wheat, Triticum aestivum (cv. Récital), were collected at 15 stages of development, from anthesis to physiological maturity, 0-700°C days (degree days after anthesis). Two hundred and seven proteins of grain peripheral layers (inner pericarp, hyaline, testa and aleurone layer) we...

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Bibliographic Details
Published in:Proteomics (Weinheim) 2011-02, Vol.11 (3), p.371-379
Main Authors: Tasleem-Tahir, Ayesha, Nadaud, Isabelle, Girousse, Christine, Martre, Pierre, Marion, Didier, Branlard, Gérard
Format: Article
Language:English
Subjects:
Agricultural sciences
Aleurone layer
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Edible Grain - growth & development
Edible Grain - metabolism
Electrophoresis, Gel, Two-Dimensional
Expression profile
Fundamental and applied biological sciences. Psychology
Grain development
Life Sciences
Miscellaneous
Peripheral layers
Plant Proteins - analysis
Plant Proteins - metabolism
Plant proteomics
Proteins
Proteome - analysis
Proteome - metabolism
Proteomics
Seeds - growth & development
Seeds - metabolism
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Triticum - growth & development
Triticum - metabolism
Triticum aestivum
Wheat
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Summary:Grains of hexaploid wheat, Triticum aestivum (cv. Récital), were collected at 15 stages of development, from anthesis to physiological maturity, 0-700°C days (degree days after anthesis). Two hundred and seven proteins of grain peripheral layers (inner pericarp, hyaline, testa and aleurone layer) were identified by 2-DE, MALDI-TOF MS and data mining, then were classified in 16 different functional categories. Study of the protein expression over time allowed identification of five main profiles and four distinct phases of development. Composite expression curves indicated that there was a shift from metabolic processes, translation, transcription and ATP interconversion towards storage and defence processes. Protein synthesis, protein turnover, signal transduction, membrane transport and biosynthesis of secondary metabolites were the mediating functions of this shift. A picture of the dynamic processes taking place in peripheral layers during grain development was obtained in this study. It should further help in the construction of proteome reference maps for the developing peripheral layers.
ISSN:1615-9853
1615-9861
1615-9861
DOI:10.1002/pmic.201000333