In Situ FT-IR Microscopic Study on Enzymatic Treatment of Poplar Wood Cross-Sections

The feasibility of Fourier transform infrared (FT-IR) microscopy to monitor in situ the enzymatic degradation of wood was investigated. Cross-sections of poplar wood were treated with cellulase Onozuka RS within a custom-built fluidic cell. Light-optical micrographs and FT-IR spectra were acquired i...

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Bibliographic Details
Published in:Biomacromolecules 2008-08, Vol.9 (8), p.2194-2201
Main Authors: Gierlinger, Notburga, Goswami, Luna, Schmidt, Martin, Burgert, Ingo, Coutand, Catherine, Rogge, Tilmann, Schwanninger, Manfred
Format: Article
Language:English
Subjects:
Agricultural sciences
Applied sciences
Biotechnology - instrumentation
Biotechnology - methods
Cellulase - chemistry
Cellulose - chemistry
Crystallization
Ethanol - chemistry
Exact sciences and technology
Fermentation
Hydrolysis
Life Sciences
Light
Optics and Photonics
Polymer industry, paints, wood
Populus - chemistry
Porosity
Properties and testing
Spectroscopy, Fourier Transform Infrared - methods
Temperature
Wood
Wood - analysis
Wood. Paper. Non wovens
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Summary:The feasibility of Fourier transform infrared (FT-IR) microscopy to monitor in situ the enzymatic degradation of wood was investigated. Cross-sections of poplar wood were treated with cellulase Onozuka RS within a custom-built fluidic cell. Light-optical micrographs and FT-IR spectra were acquired in situ from normal and tension wood fibers. Light-optical micrographs showed almost complete removal of the gelatinous (G) layer in tension wood. No structural and spectral changes were observed in the lignified cell walls. The accessibility of cellulose within the lignified cell wall was found to be the main limiting factor, whereas the depletion of the enzyme due to lignin adsorption could be ruled out. The fast, selective hydrolysis of the crystalline cellulose in the G-layer, even at room temperature, might be explained by the gel-like structure and the highly porous surface. Young plantation grown hardwood trees with a high proportion of G-fibers thus represent an interesting resource for bioconversion to fermentable sugars in the process to bioethanol.
ISSN:1525-7797
1526-4602
DOI:10.1021/bm800300b