Transcriptome analysis of muscle in horses suffering from recurrent exertional rhabdomyolysis revealed energetic pathway alterations and disruption in the cytosolic calcium regulation

Summary Recurrent exertional rhabdomyolysis (RER) is frequently observed in race horses like trotters. Some predisposing genetic factors have been described in epidemiological studies. However, the exact aetiology is still unknown. A calcium homeostasis disruption was suspected in previous experimen...

Full description

Saved in:
Bibliographic Details
Published in:Animal genetics 2012-06, Vol.43 (3), p.271-281
Main Authors: Barrey, E., Jayr, L., Mucher, E., Gospodnetic, S., Joly, F., Benech, P., Alibert, O., Gidrol, X., Mata, X., Vaiman, A., Guérin, G.
Format: Article
Language:English
Subjects:
Animals
Biochemistry, Molecular Biology
Biopsy
Calcium
Calcium - metabolism
energetics
Female
Gene expression
Gene Expression Profiling
Gene Expression Regulation
Genomics
Homeostasis
horse
Horse Diseases
Horse Diseases - genetics
Horse Diseases - physiopathology
Horses
Life Sciences
Male
Mice
Microarray Analysis
Microarray Analysis - veterinary
mitochondria
Muscle, Skeletal
Muscle, Skeletal - physiopathology
myopathy
Real-Time Polymerase Chain Reaction
Real-Time Polymerase Chain Reaction - veterinary
Rhabdomyolysis
Rhabdomyolysis - genetics
Rhabdomyolysis - physiopathology
Rhabdomyolysis - veterinary
Rodents
Transcriptome
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Summary Recurrent exertional rhabdomyolysis (RER) is frequently observed in race horses like trotters. Some predisposing genetic factors have been described in epidemiological studies. However, the exact aetiology is still unknown. A calcium homeostasis disruption was suspected in previous experimental studies, and we suggested that a transcriptome analysis of RER muscles would be a possible way to investigate the pathway disorder. The purpose of this study was to compare the gene expression profile of RER vs. control muscles in the French Trotter to determine any metabolic or structural disruption. Total RNA was extracted from the gluteal medius and longissimus lumborum muscles after biopsies in 15 French Trotter horses, including 10 controls and 5 RER horses affected by ‘tying‐up’ with high plasmatic muscular enzyme activities. Gene expression analysis was performed on the muscle biopsies using a 25K oligonucleotide microarray, which consisted of 24 009 mouse and 384 horse probes. Transcriptome analysis revealed 191 genes significantly modulated in RER vs. control muscles (P 
ISSN:0268-9146
1365-2052
DOI:10.1111/j.1365-2052.2011.02246.x