Protein expression is increased by a class III AU-rich element and tethered CUG-BP1

In mammalian somatic cells, the post-transcriptional control of cytokine or proto-oncogene expression is often achieved by factors binding to sequence elements in the 3′ untranslated region (3′UTR). The most studied are the AU-rich elements (ARE) that have been divided into three classes. Here, we s...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2006-09, Vol.347 (3), p.723-730
Main Authors: Barreau, Carine, Watrin, Tanguy, Beverley Osborne, H., Paillard, Luc
Format: Article
Language:English
Subjects:
3' Untranslated Regions
3' Untranslated Regions - classification
3' Untranslated Regions - genetics
Adenosine
Adenosine - genetics
Animals
AU-rich element (ARE)
Biochemistry, Molecular Biology
c-jun
Cell Line
Embryo deadenylation element-binding protein (EDEN-BP)
Gene Expression
Genes, Reporter
Genes, Reporter - genetics
Humans
Life Sciences
Mice
Molecular biology
Post-transcriptional control
Protein Binding
Proto-Oncogene Proteins c-jun
Proto-Oncogene Proteins c-jun - genetics
Proto-Oncogene Proteins c-jun - metabolism
RNA-Binding Proteins
RNA-Binding Proteins - genetics
RNA-Binding Proteins - metabolism
Translation
Uridine
Uridine - genetics
Xenopus laevis
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Summary:In mammalian somatic cells, the post-transcriptional control of cytokine or proto-oncogene expression is often achieved by factors binding to sequence elements in the 3′ untranslated region (3′UTR). The most studied are the AU-rich elements (ARE) that have been divided into three classes. Here, we show that in mammalian cells, the presence of the class III c-jun ARE in the 3′UTR of a reporter mRNA enhanced reporter protein expression. In contrast, the presence of a class II ARE in the 3′UTR decreased reporter protein expression. CUG-BP1/CELF1 is able to bind c-jun ARE. Protein expression was enhanced similarly to what was observed for c-jun ARE when the reporter mRNA contained a synthetic CUG-BP1/CELF1-binding site, or when this protein was tethered to the 3′UTR of a reporter mRNA. These results reveal an unexpected complexity of ARE-mediated post-transcriptional regulations, and indicate a function for CUG-BP1/CELF1 in class III ARE directed regulations.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2006.06.177