Immunolocalization of Progesterone Receptors in the Canine Oviduct around Ovulation

Contents In the bitch, oocyte maturation, sperm storage, fertilization and early embryo development take place within the oviducts under high and increasing circulating progesterone concentrations. To investigate the potential effects of progesterone on the canine oviduct, nuclear progesterone recep...

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Bibliographic Details
Published in:Reproduction in domestic animals 2012-12, Vol.47 (s6), p.35-39
Main Authors: Tahir, MZ, Reynaud, K, Mawa, G, Thoumire, S, Chastant-Maillard, S, Saint-Dizier, M
Format: Article
Language:English
Subjects:
Animal biology
Animal reproduction
Animals
Dogs
Dogs - physiology
Female
Immunohistochemistry - veterinary
Immunology
Life Sciences
Oviducts - physiology
Ovulation - physiology
Receptors, Progesterone - physiology
Reproductive Biology
Sexual reproduction
Veterinary medicine and animal Health
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Summary:Contents In the bitch, oocyte maturation, sperm storage, fertilization and early embryo development take place within the oviducts under high and increasing circulating progesterone concentrations. To investigate the potential effects of progesterone on the canine oviduct, nuclear progesterone receptors (PR) were localized. Oviducts were collected by ovariectomy from adult Beagle bitches during anestrus, after the onset of proestrus but prior to the Luteinizing Hormone (LH) peak (Pre‐LH), after the LH peak but before ovulation (Pre‐ov) and on Days 1, 4 and 7 after ovulation (n = 3 bitches per stage). The cellular distribution of PR was studied by immunohistochemistry (IHC) in the ampulla, isthmus and tubal part of the utero‐tubal junction (UTJ). Plasma progesterone and 17β‐oestradiol were assayed on the day of surgery. PR were specifically expressed in the nuclei of epithelial, stromal and muscular cells in the ampulla, isthmus and UTJ. The IHC scores did not vary from one oviductal region to another. However, the epithelium displayed higher scores than the stroma at anestrus, Pre‐ov, Days 4 and 7, and also higher scores than muscle at Days 4 and 7 (p 
ISSN:0936-6768
1439-0531
DOI:10.1111/rda.12070