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Immunolocalization of Progesterone Receptors in the Canine Oviduct around Ovulation
Contents In the bitch, oocyte maturation, sperm storage, fertilization and early embryo development take place within the oviducts under high and increasing circulating progesterone concentrations. To investigate the potential effects of progesterone on the canine oviduct, nuclear progesterone recep...
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| Published in: | Reproduction in domestic animals 2012-12, Vol.47 (s6), p.35-39 |
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| cites | cdi_FETCH-LOGICAL-c4600-f60bc34be1b80b1fe57950dd85cd25fcd3bd26d1e38ee595ddbb10efc31e0c1e3 |
| container_end_page | 39 |
| container_issue | s6 |
| container_start_page | 35 |
| container_title | Reproduction in domestic animals |
| container_volume | 47 |
| creator | Tahir, MZ Reynaud, K Mawa, G Thoumire, S Chastant-Maillard, S Saint-Dizier, M |
| description | Contents
In the bitch, oocyte maturation, sperm storage, fertilization and early embryo development take place within the oviducts under high and increasing circulating progesterone concentrations. To investigate the potential effects of progesterone on the canine oviduct, nuclear progesterone receptors (PR) were localized. Oviducts were collected by ovariectomy from adult Beagle bitches during anestrus, after the onset of proestrus but prior to the Luteinizing Hormone (LH) peak (Pre‐LH), after the LH peak but before ovulation (Pre‐ov) and on Days 1, 4 and 7 after ovulation (n = 3 bitches per stage). The cellular distribution of PR was studied by immunohistochemistry (IHC) in the ampulla, isthmus and tubal part of the utero‐tubal junction (UTJ). Plasma progesterone and 17β‐oestradiol were assayed on the day of surgery. PR were specifically expressed in the nuclei of epithelial, stromal and muscular cells in the ampulla, isthmus and UTJ. The IHC scores did not vary from one oviductal region to another. However, the epithelium displayed higher scores than the stroma at anestrus, Pre‐ov, Days 4 and 7, and also higher scores than muscle at Days 4 and 7 (p |
| doi_str_mv | 10.1111/rda.12070 |
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In the bitch, oocyte maturation, sperm storage, fertilization and early embryo development take place within the oviducts under high and increasing circulating progesterone concentrations. To investigate the potential effects of progesterone on the canine oviduct, nuclear progesterone receptors (PR) were localized. Oviducts were collected by ovariectomy from adult Beagle bitches during anestrus, after the onset of proestrus but prior to the Luteinizing Hormone (LH) peak (Pre‐LH), after the LH peak but before ovulation (Pre‐ov) and on Days 1, 4 and 7 after ovulation (n = 3 bitches per stage). The cellular distribution of PR was studied by immunohistochemistry (IHC) in the ampulla, isthmus and tubal part of the utero‐tubal junction (UTJ). Plasma progesterone and 17β‐oestradiol were assayed on the day of surgery. PR were specifically expressed in the nuclei of epithelial, stromal and muscular cells in the ampulla, isthmus and UTJ. The IHC scores did not vary from one oviductal region to another. However, the epithelium displayed higher scores than the stroma at anestrus, Pre‐ov, Days 4 and 7, and also higher scores than muscle at Days 4 and 7 (p < 0.05). Immunohistochemistry scores in the stroma and muscle decreased at Days 4 and 7 compared with previous stages (p < 0.05). Furthermore, muscular IHC scores were positively correlated with circulating 17β‐oestradiol concentrations and negatively correlated with circulating progesterone concentrations (p < 0.05). In conclusion, PR were identified in the canine oviduct, with differences in expression between tissues and times around ovulation, suggesting that progesterone may regulate tubal functions and reproductive events in this species.</description><identifier>ISSN: 0936-6768</identifier><identifier>EISSN: 1439-0531</identifier><identifier>DOI: 10.1111/rda.12070</identifier><identifier>PMID: 23279461</identifier><language>eng</language><publisher>Germany: Blackwell Publishing Ltd</publisher><subject>Animal biology ; Animal reproduction ; Animals ; Dogs ; Dogs - physiology ; Female ; Immunohistochemistry - veterinary ; Immunology ; Life Sciences ; Oviducts - physiology ; Ovulation - physiology ; Receptors, Progesterone - physiology ; Reproductive Biology ; Sexual reproduction ; Veterinary medicine and animal Health</subject><ispartof>Reproduction in domestic animals, 2012-12, Vol.47 (s6), p.35-39</ispartof><rights>2012 Blackwell Verlag GmbH</rights><rights>2012 Blackwell Verlag GmbH.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4600-f60bc34be1b80b1fe57950dd85cd25fcd3bd26d1e38ee595ddbb10efc31e0c1e3</citedby><cites>FETCH-LOGICAL-c4600-f60bc34be1b80b1fe57950dd85cd25fcd3bd26d1e38ee595ddbb10efc31e0c1e3</cites><orcidid>0000-0002-4569-5665 ; 0000-0003-0790-6377 ; 0000-0002-8897-6790</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23279461$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-01000422$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Tahir, MZ</creatorcontrib><creatorcontrib>Reynaud, K</creatorcontrib><creatorcontrib>Mawa, G</creatorcontrib><creatorcontrib>Thoumire, S</creatorcontrib><creatorcontrib>Chastant-Maillard, S</creatorcontrib><creatorcontrib>Saint-Dizier, M</creatorcontrib><title>Immunolocalization of Progesterone Receptors in the Canine Oviduct around Ovulation</title><title>Reproduction in domestic animals</title><addtitle>Reprod Domest Anim</addtitle><description>Contents
In the bitch, oocyte maturation, sperm storage, fertilization and early embryo development take place within the oviducts under high and increasing circulating progesterone concentrations. To investigate the potential effects of progesterone on the canine oviduct, nuclear progesterone receptors (PR) were localized. Oviducts were collected by ovariectomy from adult Beagle bitches during anestrus, after the onset of proestrus but prior to the Luteinizing Hormone (LH) peak (Pre‐LH), after the LH peak but before ovulation (Pre‐ov) and on Days 1, 4 and 7 after ovulation (n = 3 bitches per stage). The cellular distribution of PR was studied by immunohistochemistry (IHC) in the ampulla, isthmus and tubal part of the utero‐tubal junction (UTJ). Plasma progesterone and 17β‐oestradiol were assayed on the day of surgery. PR were specifically expressed in the nuclei of epithelial, stromal and muscular cells in the ampulla, isthmus and UTJ. The IHC scores did not vary from one oviductal region to another. However, the epithelium displayed higher scores than the stroma at anestrus, Pre‐ov, Days 4 and 7, and also higher scores than muscle at Days 4 and 7 (p < 0.05). Immunohistochemistry scores in the stroma and muscle decreased at Days 4 and 7 compared with previous stages (p < 0.05). Furthermore, muscular IHC scores were positively correlated with circulating 17β‐oestradiol concentrations and negatively correlated with circulating progesterone concentrations (p < 0.05). In conclusion, PR were identified in the canine oviduct, with differences in expression between tissues and times around ovulation, suggesting that progesterone may regulate tubal functions and reproductive events in this species.</description><subject>Animal biology</subject><subject>Animal reproduction</subject><subject>Animals</subject><subject>Dogs</subject><subject>Dogs - physiology</subject><subject>Female</subject><subject>Immunohistochemistry - veterinary</subject><subject>Immunology</subject><subject>Life Sciences</subject><subject>Oviducts - physiology</subject><subject>Ovulation - physiology</subject><subject>Receptors, Progesterone - physiology</subject><subject>Reproductive Biology</subject><subject>Sexual reproduction</subject><subject>Veterinary medicine and animal Health</subject><issn>0936-6768</issn><issn>1439-0531</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNp1kU9v1DAQxS0EokvhwBdAkbjAIe1MHMfJcbXQbaUVrbYFjpZjT6hLEi9O0j98etxuu0hIzMWap9-88egx9hbhAGMdBqsPMAMJz9gMc16lIDg-ZzOoeJEWsij32KthuAJAUUr5ku1lPJNVXuCMnZ903dT71hvdut96dL5PfJOcBf-DhpGC7ylZk6HN6MOQuD4ZLylZ6N5F_fTa2cmMiQ5-6m1sp_bB4DV70eh2oDeP7z77evT5YnGcrk6XJ4v5KjV5AZA2BdSG5zVhXUKNDQlZCbC2FMZmojGW1zYrLBIviUQlrK1rBGoMRwIT5X32cet7qVu1Ca7T4U557dTxfKXuNUAAyLPsGiP7Yctugv81xdNU5wZDbat78tOgMJMcC8xFGdH3_6BXfgp9vCRSeVaWQmL1d7kJfhgCNbsfIKj7VFRMRT2kEtl3j45T3ZHdkU8xROBwC9y4lu7-76TWn-ZPlul2wsWUbncTOvxUheRSqO9fluroolyuv-G5OuN_ANuApT8</recordid><startdate>201212</startdate><enddate>201212</enddate><creator>Tahir, MZ</creator><creator>Reynaud, K</creator><creator>Mawa, G</creator><creator>Thoumire, S</creator><creator>Chastant-Maillard, S</creator><creator>Saint-Dizier, M</creator><general>Blackwell Publishing Ltd</general><general>Wiley</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-4569-5665</orcidid><orcidid>https://orcid.org/0000-0003-0790-6377</orcidid><orcidid>https://orcid.org/0000-0002-8897-6790</orcidid></search><sort><creationdate>201212</creationdate><title>Immunolocalization of Progesterone Receptors in the Canine Oviduct around Ovulation</title><author>Tahir, MZ ; Reynaud, K ; Mawa, G ; Thoumire, S ; Chastant-Maillard, S ; Saint-Dizier, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4600-f60bc34be1b80b1fe57950dd85cd25fcd3bd26d1e38ee595ddbb10efc31e0c1e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animal biology</topic><topic>Animal reproduction</topic><topic>Animals</topic><topic>Dogs</topic><topic>Dogs - physiology</topic><topic>Female</topic><topic>Immunohistochemistry - veterinary</topic><topic>Immunology</topic><topic>Life Sciences</topic><topic>Oviducts - physiology</topic><topic>Ovulation - physiology</topic><topic>Receptors, Progesterone - physiology</topic><topic>Reproductive Biology</topic><topic>Sexual reproduction</topic><topic>Veterinary medicine and animal Health</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tahir, MZ</creatorcontrib><creatorcontrib>Reynaud, K</creatorcontrib><creatorcontrib>Mawa, G</creatorcontrib><creatorcontrib>Thoumire, S</creatorcontrib><creatorcontrib>Chastant-Maillard, S</creatorcontrib><creatorcontrib>Saint-Dizier, M</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Reproduction in domestic animals</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tahir, MZ</au><au>Reynaud, K</au><au>Mawa, G</au><au>Thoumire, S</au><au>Chastant-Maillard, S</au><au>Saint-Dizier, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunolocalization of Progesterone Receptors in the Canine Oviduct around Ovulation</atitle><jtitle>Reproduction in domestic animals</jtitle><addtitle>Reprod Domest Anim</addtitle><date>2012-12</date><risdate>2012</risdate><volume>47</volume><issue>s6</issue><spage>35</spage><epage>39</epage><pages>35-39</pages><issn>0936-6768</issn><eissn>1439-0531</eissn><abstract>Contents
In the bitch, oocyte maturation, sperm storage, fertilization and early embryo development take place within the oviducts under high and increasing circulating progesterone concentrations. To investigate the potential effects of progesterone on the canine oviduct, nuclear progesterone receptors (PR) were localized. Oviducts were collected by ovariectomy from adult Beagle bitches during anestrus, after the onset of proestrus but prior to the Luteinizing Hormone (LH) peak (Pre‐LH), after the LH peak but before ovulation (Pre‐ov) and on Days 1, 4 and 7 after ovulation (n = 3 bitches per stage). The cellular distribution of PR was studied by immunohistochemistry (IHC) in the ampulla, isthmus and tubal part of the utero‐tubal junction (UTJ). Plasma progesterone and 17β‐oestradiol were assayed on the day of surgery. PR were specifically expressed in the nuclei of epithelial, stromal and muscular cells in the ampulla, isthmus and UTJ. The IHC scores did not vary from one oviductal region to another. However, the epithelium displayed higher scores than the stroma at anestrus, Pre‐ov, Days 4 and 7, and also higher scores than muscle at Days 4 and 7 (p < 0.05). Immunohistochemistry scores in the stroma and muscle decreased at Days 4 and 7 compared with previous stages (p < 0.05). Furthermore, muscular IHC scores were positively correlated with circulating 17β‐oestradiol concentrations and negatively correlated with circulating progesterone concentrations (p < 0.05). In conclusion, PR were identified in the canine oviduct, with differences in expression between tissues and times around ovulation, suggesting that progesterone may regulate tubal functions and reproductive events in this species.</abstract><cop>Germany</cop><pub>Blackwell Publishing Ltd</pub><pmid>23279461</pmid><doi>10.1111/rda.12070</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0002-4569-5665</orcidid><orcidid>https://orcid.org/0000-0003-0790-6377</orcidid><orcidid>https://orcid.org/0000-0002-8897-6790</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Reproduction in domestic animals, 2012-12, Vol.47 (s6), p.35-39 |
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| language | eng |
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| source | Wiley-Blackwell Read & Publish Collection |
| subjects | Animal biology Animal reproduction Animals Dogs Dogs - physiology Female Immunohistochemistry - veterinary Immunology Life Sciences Oviducts - physiology Ovulation - physiology Receptors, Progesterone - physiology Reproductive Biology Sexual reproduction Veterinary medicine and animal Health |
| title | Immunolocalization of Progesterone Receptors in the Canine Oviduct around Ovulation |
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