Phenotypic and genetic characterization of Chilean isolates of Botrytis cinerea with different levels of sensitivity to fenhexamid

Forty three Chilean Botrytis cinerea isolates of different fenhexamid sensitivities, obtained from table grapes, were phenotypically analyzed and sequenced for the erg27 gene that encodes the 3-ketoreductase enzyme. Fifteen isolates were highly resistant to fenhexamid (HydR3⁺) with conidial germinat...

Full description

Saved in:
Bibliographic Details
Published in:Phytopathologia mediterranea 2011-12, Vol.50 (3), p.414-420
Main Authors: ESTERIO, Marcela, RAMOS, Cecilia, WALKER, Anne-Sophie, FILLINGER, Sabine, LEROUX, Pierre, AUGER, Jaime
Format: Article
Language:English
Subjects:
Agricultural sciences
Amino acid sequence
Amino acids
Augers
Benzimidazole
Benzimidazoles
Botrytis
Botrytis cinerea
Colonies
Dicarboximide
DNA sequence analysis
Enzymes
fenhexamid
Fungicides
Genetic mutation
Germination
Life Sciences
Phenotypes
Phytopathology
RESEARCH PAPERS
Transmembrane domains
Vitaceae
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Forty three Chilean Botrytis cinerea isolates of different fenhexamid sensitivities, obtained from table grapes, were phenotypically analyzed and sequenced for the erg27 gene that encodes the 3-ketoreductase enzyme. Fifteen isolates were highly resistant to fenhexamid (HydR3⁺) with conidial germination EC₅₀ values >5 μg·mL⁻¹ and colony growth EC50 values >2 μg·mL⁻¹. Five isolates had slight to moderate resistance levels (HydR3⁻) with conidial germination EC50 values between 0.7 and 2.6 μg·mL⁻¹ and colony growth EC50 values between 0.4 and 3 μg·mL⁻¹. Twenty-three isolates were fenhexamid sensitive (HydS) (conidial germination and colony growth EC50 values
ISSN:0031-9465
1593-2095