Effect of blood handling conditions on progesterone assay results obtained by chemiluminescence in the bitch

Assay of blood progesterone (P4) is commonly practiced to determine the time of ovulation, diagnose luteal insufficiency, and predict time of parturition in bitches. Because of practical constraints, most blood samples cannot be assayed on site immediately after collection. The aim of this work was...

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Bibliographic Details
Published in:Domestic animal endocrinology 2013-10, Vol.45 (3), p.141-144
Main Authors: Tahir, M.Z., Thoumire, S., Raffaelli, M., Grimard, B., Reynaud, K., Chastant-Maillard, S.
Format: Article
Language:English
Subjects:
Animals
Anticoagulant
Anticoagulants
Blood Preservation - veterinary
Blood Specimen Collection - instrumentation
Blood Specimen Collection - methods
Blood Specimen Collection - veterinary
Canine
Chemiluminescence
Cryopreservation - veterinary
Development Biology
Dogs - blood
Drug Stability
Female
Freeze-thaw cycles
Immunoassay - methods
Immunoassay - veterinary
Life Sciences
Luminescent Measurements - veterinary
Progesterone
Progesterone - blood
Specimen Handling - instrumentation
Specimen Handling - methods
Storage time
Time Factors
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Summary:Assay of blood progesterone (P4) is commonly practiced to determine the time of ovulation, diagnose luteal insufficiency, and predict time of parturition in bitches. Because of practical constraints, most blood samples cannot be assayed on site immediately after collection. The aim of this work was to evaluate the effects of various sampling and storage conditions on concentrations of P4 as determined by chemiluminescence immunoassay. The blood of 5 Beagle bitches was collected from the jugular vein to study the effect of the type of collection tube (silicone, lithium heparin, EDTA), the storage time of unseparated or separated plasma (2 h to 14 d), and the number of freeze-thaw cycles (1–10) on P4. The effect of each factor was tested within one assay session. None of the factors significantly affected P4. Thus, P4 appears to remain relatively stable in canine blood samples exposed to various processing and storage conditions.
ISSN:0739-7240
1879-0054
DOI:10.1016/j.domaniend.2013.07.002