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Effect of blood handling conditions on progesterone assay results obtained by chemiluminescence in the bitch
Assay of blood progesterone (P4) is commonly practiced to determine the time of ovulation, diagnose luteal insufficiency, and predict time of parturition in bitches. Because of practical constraints, most blood samples cannot be assayed on site immediately after collection. The aim of this work was...
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| Published in: | Domestic animal endocrinology 2013-10, Vol.45 (3), p.141-144 |
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| Main Authors: | , , , , , |
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| cited_by | cdi_FETCH-LOGICAL-c405t-6d814d1d0e8322d534d05bc4eea211d914909df991a912ac2252476917b242f3 |
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| cites | cdi_FETCH-LOGICAL-c405t-6d814d1d0e8322d534d05bc4eea211d914909df991a912ac2252476917b242f3 |
| container_end_page | 144 |
| container_issue | 3 |
| container_start_page | 141 |
| container_title | Domestic animal endocrinology |
| container_volume | 45 |
| creator | Tahir, M.Z. Thoumire, S. Raffaelli, M. Grimard, B. Reynaud, K. Chastant-Maillard, S. |
| description | Assay of blood progesterone (P4) is commonly practiced to determine the time of ovulation, diagnose luteal insufficiency, and predict time of parturition in bitches. Because of practical constraints, most blood samples cannot be assayed on site immediately after collection. The aim of this work was to evaluate the effects of various sampling and storage conditions on concentrations of P4 as determined by chemiluminescence immunoassay. The blood of 5 Beagle bitches was collected from the jugular vein to study the effect of the type of collection tube (silicone, lithium heparin, EDTA), the storage time of unseparated or separated plasma (2 h to 14 d), and the number of freeze-thaw cycles (1–10) on P4. The effect of each factor was tested within one assay session. None of the factors significantly affected P4. Thus, P4 appears to remain relatively stable in canine blood samples exposed to various processing and storage conditions. |
| doi_str_mv | 10.1016/j.domaniend.2013.07.002 |
| format | article |
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Because of practical constraints, most blood samples cannot be assayed on site immediately after collection. The aim of this work was to evaluate the effects of various sampling and storage conditions on concentrations of P4 as determined by chemiluminescence immunoassay. The blood of 5 Beagle bitches was collected from the jugular vein to study the effect of the type of collection tube (silicone, lithium heparin, EDTA), the storage time of unseparated or separated plasma (2 h to 14 d), and the number of freeze-thaw cycles (1–10) on P4. The effect of each factor was tested within one assay session. None of the factors significantly affected P4. Thus, P4 appears to remain relatively stable in canine blood samples exposed to various processing and storage conditions.</description><identifier>ISSN: 0739-7240</identifier><identifier>EISSN: 1879-0054</identifier><identifier>DOI: 10.1016/j.domaniend.2013.07.002</identifier><identifier>PMID: 23988180</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Anticoagulant ; Anticoagulants ; Blood Preservation - veterinary ; Blood Specimen Collection - instrumentation ; Blood Specimen Collection - methods ; Blood Specimen Collection - veterinary ; Canine ; Chemiluminescence ; Cryopreservation - veterinary ; Development Biology ; Dogs - blood ; Drug Stability ; Female ; Freeze-thaw cycles ; Immunoassay - methods ; Immunoassay - veterinary ; Life Sciences ; Luminescent Measurements - veterinary ; Progesterone ; Progesterone - blood ; Specimen Handling - instrumentation ; Specimen Handling - methods ; Storage time ; Time Factors</subject><ispartof>Domestic animal endocrinology, 2013-10, Vol.45 (3), p.141-144</ispartof><rights>2013 Elsevier Inc.</rights><rights>Copyright © 2013 Elsevier Inc. 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Thus, P4 appears to remain relatively stable in canine blood samples exposed to various processing and storage conditions.</description><subject>Animals</subject><subject>Anticoagulant</subject><subject>Anticoagulants</subject><subject>Blood Preservation - veterinary</subject><subject>Blood Specimen Collection - instrumentation</subject><subject>Blood Specimen Collection - methods</subject><subject>Blood Specimen Collection - veterinary</subject><subject>Canine</subject><subject>Chemiluminescence</subject><subject>Cryopreservation - veterinary</subject><subject>Development Biology</subject><subject>Dogs - blood</subject><subject>Drug Stability</subject><subject>Female</subject><subject>Freeze-thaw cycles</subject><subject>Immunoassay - methods</subject><subject>Immunoassay - veterinary</subject><subject>Life Sciences</subject><subject>Luminescent Measurements - veterinary</subject><subject>Progesterone</subject><subject>Progesterone - blood</subject><subject>Specimen Handling - instrumentation</subject><subject>Specimen Handling - methods</subject><subject>Storage time</subject><subject>Time Factors</subject><issn>0739-7240</issn><issn>1879-0054</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNqFkc1u3CAUhVHVqpmmfYWWZbuwe8F4MMtRlDaVRuome4ThOmZkQwp2pHn7Yk06264QcM65Px8hXxjUDNj--6l2cTbBY3A1B9bUIGsA_obsWCdVBdCKt2QHslGV5AJuyIecTwAgi_s9ueGN6jrWwY5M98OAdqFxoP0Uo6OjCW7y4YnaGJxffAyZxkCfU3zCvGCKAanJ2ZxpwrxOS_ntF-MDOtqfqR1x9tM6l3u2GCxSH-gyIu39YseP5N1gpoyfXs9b8vjj_vHuoTr-_vnr7nCsrIB2qfauY8IxB9g1nLu2EQ7a3gpEwxlzigkFyg1KMaMYN5bzlgu5V0z2XPChuSXfLrGjmfRz8rNJZx2N1w-Ho97egAEI0XYvrGi_XrRlwD9rmVDPvnQ-TSZgXLNmohFSSNZtUnmR2hRzTjhcsxnojYo-6SsVvVHRIHWhUpyfX4us_Yzu6vuHoQgOFwGWrbx4TDpbv63P-VTolFj_3yJ_Aa-ioiA</recordid><startdate>20131001</startdate><enddate>20131001</enddate><creator>Tahir, M.Z.</creator><creator>Thoumire, S.</creator><creator>Raffaelli, M.</creator><creator>Grimard, B.</creator><creator>Reynaud, K.</creator><creator>Chastant-Maillard, S.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-5419-4774</orcidid><orcidid>https://orcid.org/0000-0003-0790-6377</orcidid><orcidid>https://orcid.org/0000-0002-8897-6790</orcidid></search><sort><creationdate>20131001</creationdate><title>Effect of blood handling conditions on progesterone assay results obtained by chemiluminescence in the bitch</title><author>Tahir, M.Z. ; 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| ispartof | Domestic animal endocrinology, 2013-10, Vol.45 (3), p.141-144 |
| issn | 0739-7240 1879-0054 |
| language | eng |
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| source | ScienceDirect Freedom Collection 2022-2024 |
| subjects | Animals Anticoagulant Anticoagulants Blood Preservation - veterinary Blood Specimen Collection - instrumentation Blood Specimen Collection - methods Blood Specimen Collection - veterinary Canine Chemiluminescence Cryopreservation - veterinary Development Biology Dogs - blood Drug Stability Female Freeze-thaw cycles Immunoassay - methods Immunoassay - veterinary Life Sciences Luminescent Measurements - veterinary Progesterone Progesterone - blood Specimen Handling - instrumentation Specimen Handling - methods Storage time Time Factors |
| title | Effect of blood handling conditions on progesterone assay results obtained by chemiluminescence in the bitch |
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