Investigating the Cell Membrane via Single Particle Tracking, Bayesian Inference and Hydrodynamic Force Application

We investigate the potential felt by membrane receptors inside membrane microdomains via tracking of single receptors labelled with rare-earth doped luminescent nanoparticles and Bayesian inference analysis of the recorded trajectories. We demonstrated that the potential felt by peptidic toxin recep...

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Bibliographic Details
Published in:Biophysical journal 2014-01, Vol.106 (2), p.633a-633a
Main Authors: Richly, Maximilian U., Türkcan, Silvan, Bouzigues, Cedric, Popoff, Michel R., Masson, Jean-Baptiste, Allain, Jean-Marc, Alexandrou, Antigoni
Format: Article
Language:English
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Summary:We investigate the potential felt by membrane receptors inside membrane microdomains via tracking of single receptors labelled with rare-earth doped luminescent nanoparticles and Bayesian inference analysis of the recorded trajectories. We demonstrated that the potential felt by peptidic toxin receptors confined in lipid rafts is well described by a second-order polynomial potential, possibly due to an inhomogeneous lipid and protein distribution [Türkcan et al., Biophys. J. 2012] In contrast, the potential experienced by transferrin receptors in cytoskeleton-delimited microdomains is localized at the border of the confinement domain.
ISSN:0006-3495
1542-0086
DOI:10.1016/j.bpj.2013.11.3504