Pulse radiolysis studies on superoxide reductase from Treponema pallidum

Superoxide reductases (SORs) are small metalloenzymes, which catalyze reduction of O 2 − to H 2O 2. The reaction of the enzyme from Treponema pallidum with superoxide was studied by pulse radiolysis methods. The first step is an extremely fast bi-molecular reaction of the ferrous center with O 2 − ,...

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Bibliographic Details
Published in:FEBS letters 2001-05, Vol.497 (2), p.171-173
Main Authors: Nivière, Vincent, Lombard, Murielle, Fontecave, Marc, Houée-Levin, Chantal
Format: Article
Language:English
Subjects:
Biochemistry
Biochemistry, Molecular Biology
Catalysis
Hydrogen Peroxide - chemistry
Iron - chemistry
Kinetics
Life Sciences
Mononuclear iron center
Oxidative stress
Oxidoreductases - chemistry
Pulse Radiolysis
Reaction intermediate
Reactive Oxygen Species
SOR, superoxide reductase
Spectrum Analysis
Superoxide reductase
Superoxides - chemistry
Treponema pallidum
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Summary:Superoxide reductases (SORs) are small metalloenzymes, which catalyze reduction of O 2 − to H 2O 2. The reaction of the enzyme from Treponema pallidum with superoxide was studied by pulse radiolysis methods. The first step is an extremely fast bi-molecular reaction of the ferrous center with O 2 − , with a rate constant of 6×10 8 M −1 s −1. A first intermediate is formed which is converted to a second one with a slower rate constant of 4800 s −1. This latter value is 10 times higher than the corresponding one previously reported in the case of SOR from Desulfoarculus baarsii. The reconstituted spectra for the two intermediates are consistent with formation of transient iron-peroxide species.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(01)02468-1