Stochastic 3D optical mapping by holographic localization of Brownian scatterers

We present a wide-field microscopy technique for the 3D mapping of optical intensity distributions using Brownian gold nanopar-ticles as local probes, which are localized by off-axis holography. Fast computation methods allow us to localize hundreds of particles per minute with accuracies as good as...

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Bibliographic Details
Published in:Optics express 2014-11, Vol.22 (23), p.29191-29203
Main Authors: Martinez-Marrades, Ariadna, Rupprecht, Jean-François, Gross, Michel, Tessier, Gilles
Format: Article
Language:English
Subjects:
Holography - methods
Light
Microscopy - methods
Optics
Physics
Scattering, Radiation
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Summary:We present a wide-field microscopy technique for the 3D mapping of optical intensity distributions using Brownian gold nanopar-ticles as local probes, which are localized by off-axis holography. Fast computation methods allow us to localize hundreds of particles per minute with accuracies as good as 3 × 3 × 10nm³ for immobilized particles. Factors limiting this accuracy are discussed and the possibilities of the technique are illustrated through the 3D optical mapping of an evanescent and a propagative wave. Our results pave the way for a new stochastic imaging technique, well adapted to subwavelength optical characterization in water-based systems.
ISSN:1094-4087
1094-4087
DOI:10.1364/OE.22.029191