Magnetic nanoparticle DNA labeling for individual bacterial cell detection and recovery

A culture independent approach was developed for recovering individual bacterial cells out of communities from complex environments including soils and sediments where autofluorescent contaminants hinder the use of fluorescence based techniques. For that purpose fifty nanometer sized streptavidin-co...

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Bibliographic Details
Published in:Journal of microbiological methods 2014-12, Vol.107, p.84-91
Main Authors: Pivetal, Jérémy, Ciuta, Georgeta, Frenea-Robin, Marie, Haddour, Naoufel, Dempsey, Nora M., Dumas-Bouchiat, Frédéric, Simonet, Pascal
Format: Article
Language:English
Subjects:
Bacteria - genetics
Bacteria - isolation & purification
DNA - chemistry
DNA, Bacterial - chemistry
Electric power
Electro-transformation
Engineering Sciences
Environmental Microbiology
Escherichia coli
Escherichia coli - genetics
Escherichia coli - isolation & purification
Horizontal gene transfer
Magnetic bacterial cell labeling
Magnetic DNA labeling
Magnetic nanoparticles
Magnetite Nanoparticles - chemistry
Magnetite Nanoparticles - toxicity
Plasmids - chemistry
Transformation, Bacterial
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Summary:A culture independent approach was developed for recovering individual bacterial cells out of communities from complex environments including soils and sediments where autofluorescent contaminants hinder the use of fluorescence based techniques. For that purpose fifty nanometer sized streptavidin-coated superparamagnetic nanoparticles were used to chemically bond biotin-functionalized plasmid DNA molecules. We show that micromagnets can efficiently trap magnetically labeled transformed Escherichia coli cells after these bacteria were subjected to electro-transformation by these nanoparticle-labeled plasmids. Among other applications, this method could extend the range of approaches developed to study DNA dissemination among environmental bacteria without requiring cultivability of recombinant strains or expression of heterologous genes in the new hosts.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2014.09.006