Genetic diversity of wild and cultivated grapevine accessions from southeast Turkey

Wild grapevine genetic diversity in southeast Turkey has not been documented to date. In the present work, in order to clarify the relationships between wild and cultivated grape accessions from southeastern Turkey, 22 nuclear and three chloroplast microsatellite loci were used on 21 wild grapevine...

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Bibliographic Details
Published in:Hereditas 2014-10, Vol.151 (4-5), p.73-80
Main Authors: Karataş, Dilek Deǧirmenci, Karataş, Hüseyin, Laucou, Valérie, Sarikamiş, Gölge, Riahi, Leila, Bacilieri, Roberto, This, Patrice
Format: Article
Language:English
Subjects:
Alleles
Breeding
DNA, Chloroplast - genetics
Gene Frequency
Genetic diversity
Genetic Markers
Genetic Variation
Genotype
Germplasm
Haplotypes
Heterozygote
Life Sciences
Microsatellite Repeats
Turkey
Vitaceae
Vitis - genetics
Vitis vinifera
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Summary:Wild grapevine genetic diversity in southeast Turkey has not been documented to date. In the present work, in order to clarify the relationships between wild and cultivated grape accessions from southeastern Turkey, 22 nuclear and three chloroplast microsatellite loci were used on 21 wild grapevine Vitis vinifera L. ssp. sylvestris (Gmelin) and 13 cultivated grapevine Vitis vinifera ssp. sativa accessions. The number of alleles per SSR locus ranged from 4 (VVIn16) to 20 (VVIv67) and the mean allele number per locus was 10.09. Expected locus heterozygosity ranged from 0.586 (locus VVIb01) to 0.898 (locus (VVIv67)). The three cpSSR molecular markers presented variation in size both in cultivars and in wild Turkish accessions. Two size variants were detected for cpSSR3 (106 and 107 bp) for cpSSR5 (104 and 105 bp), and for cpSSR10 (115 and 116 bp). The six alleles in wild grapevines fell into three haplotypes B, C and D. A genetic structure according to accessions taxonomic status (wild or cultivated) was revealed by UPGMA analysis. This highlighted a clear separation between domesticated and wild accessions in Turkish germplasm. The results pointed out the need to further collect and characterize this wild and cultivated grapevine germplasm.
ISSN:0018-0661
1601-5223
DOI:10.1111/hrd2.00039