Differential expression analysis of a subset of GmNAC genes in shoots of two contrasting drought-responsive soybean cultivars DT51 and MTD720 under normal and drought conditions

NAC transcription factors are known to be involved in regulation of plant responses to drought stress. In this study, the expression of 23 drought-responsive GmNAC genes was assessed in the shoot tissues of DT51 and MTD720, the two soybean varieties with contrasting drought-responsive phenotypes, by...

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Bibliographic Details
Published in:Molecular biology reports 2014-09, Vol.41 (9), p.5563-5569
Main Authors: Thu, Nguyen Binh Anh, Hoang, Xuan Lan Thi, Doan, Hieu, Nguyen, Thanh-Hao, Bui, Dao, Thao, Nguyen Phuong, Phan Tran, Lam-Son
Format: Article
Language:English
Subjects:
Animal Anatomy
Animal Biochemistry
Biomedical and Life Sciences
Cultivars
Drought
drought tolerance
Droughts
Gene expression
gene expression regulation
Gene Expression Regulation, Plant
genes
Genes, Plant
genetic engineering
Genotype
Glycine max - classification
Glycine max - genetics
Histology
Life Sciences
Morphology
Multigene Family
Phenotype
plant response
quantitative polymerase chain reaction
Real-Time Polymerase Chain Reaction
RNA, Plant - genetics
shoots
Soybeans
Stress, Physiological
transcription factors
Transcription Factors - genetics
Up-Regulation
Vegetal Biology
water stress
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Summary:NAC transcription factors are known to be involved in regulation of plant responses to drought stress. In this study, the expression of 23 drought-responsive GmNAC genes was assessed in the shoot tissues of DT51 and MTD720, the two soybean varieties with contrasting drought-responsive phenotypes, by real-time quantitative PCR (RT-qPCR) under normal and drought conditions. Results indicated that expression profile of GmNAC genes was genotype-dependent, and six GmNACs (GmNAC019, 043, 062, 085, 095 and 101) had higher transcript levels in the shoots of the drought-tolerant DT51 in comparison with the drought-sensitive MTD720 under drought. Our study suggests a positive correlation between the higher drought tolerance degree of DT51 versus MTD720 and the up-regulation of at least these six drought-responsive GmNACs in the shoot tissues. Furthermore, on the basis of our analysis, three genes, GmNAC043, 085 and 101, were identified as promising candidates for development of drought-tolerant soybean cultivars by genetic engineering.
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-014-3507-9