Transfection of isolated rainbow trout, Oncorhynchus mykiss , granulosa cells through chemical transfection and electroporation at 12 °C

Highlights • Rainbow trout granulosa cells were isolated and cultured in vitro. • Electroporation is more efficient on granulosa cells than chemical transfection. • Electroporation by itself can have a strong impact on granulosa cells steroidogenesis.

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Bibliographic Details
Published in:General and comparative endocrinology 2015-09, Vol.221, p.165-172
Main Authors: Marivin, E, Mourot, B, Loyer, P, Rime, H, Bobe, J, Fostier, A
Format: Article
Language:English
Subjects:
Animals
Cells, Cultured
Electroporation
Electroporation - methods
Endocrinology & Metabolism
Estradiol - metabolism
Female
Gene Transfer Techniques
Granulosa
Granulosa Cells - cytology
Granulosa Cells - metabolism
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
Humans
Life Sciences
Oncorhynchus mykiss - growth & development
Oncorhynchus mykiss - metabolism
Plasmids - administration & dosage
Primary culture of cells
Radioimmunoassay
Rainbow trout
Transfection
Transfection - methods
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Description
Summary:Highlights • Rainbow trout granulosa cells were isolated and cultured in vitro. • Electroporation is more efficient on granulosa cells than chemical transfection. • Electroporation by itself can have a strong impact on granulosa cells steroidogenesis.
ISSN:0016-6480
1095-6840
DOI:10.1016/j.ygcen.2015.03.012