Reduction of adhesion properties of Ruditapes philippinarum hemocytes exposed to Vibrio tapetis

Vibrio tapetis is the causative agent of brown ring disease (BRD), which affects a species of clam, Ruditapes philippinarum. After incubation with V. tapetis, hemocytes lose filopods and become rounded, indicating cytotoxic activity of the bacterium. To rapidly quantify this cytotoxicity, a flow-cyt...

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Bibliographic Details
Published in:Diseases of aquatic organisms 2003-12, Vol.57 (1-2), p.109-116
Main Authors: CHOQUET, Gwénaëlle, SOUDANT, Philippe, LAMBERT, Christophe, NICOLAS, Jean-Louis, PAILLARD, Christine
Format: Article
Language:English
Subjects:
Agricultural sciences
Analysis of Variance
Animal aquaculture
Animal biology
Animal productions
Animals
Biodiversity and Ecology
Biological and medical sciences
Bivalvia
Bivalvia - metabolism
Bivalvia - microbiology
Cell Adhesion
Ecology, environment
Ecosystems
Environmental Sciences
Europe
Flow Cytometry
Fundamental and applied biological sciences. Psychology
Global Changes
Hemocytes
Hemocytes - metabolism
Invertebrate aquaculture
Invertebrate Zoology
Invertebrates
Life Sciences
Marine
Mollusca
Pathology
Ruditapes philippinarum
Sciences and technics of fishery
Seawater
Sensitivity and Specificity
Symbiosis
Veterinary medicine and animal Health
Vibrio
Vibrio - pathogenicity
Vibrio tapetis
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Summary:Vibrio tapetis is the causative agent of brown ring disease (BRD), which affects a species of clam, Ruditapes philippinarum. After incubation with V. tapetis, hemocytes lose filopods and become rounded, indicating cytotoxic activity of the bacterium. To rapidly quantify this cytotoxicity, a flow-cytometry test was developed based on the capacity of V. tapetis to inhibit adhesion of clam hemocytes to plastic. Several bacteria:hemocyte ratios, the cytotoxicity of other Vibrio spp. pathogenic to bivalves, and that of various V. tapetis isolates were tested. Inhibition of adherence is detectable with as few as 5 bacteria per hemocyte. The greater cytotoxic activity of V. tapetis compared to that of V. splendidus and V. pectenicida suggests a specific pathogenicity of V. tapetis to R. philippinarum hemocytes. Although all V. tapetis isolates inhibited adhesion, significant variations in cytotoxicity among isolates was demonstrated.
ISSN:0177-5103
1616-1580
DOI:10.3354/dao057109