Relationship between severity of blackleg (Leptosphaeria maculans/L. biglobosa species complex) and subsequent primary inoculum production on oilseed rape stubble

• Published in: Plant pathology 2009-02, Vol.58 (1), p.61-70
• Main Authors: Lô‐Pelzer, E., Aubertot, J. N., David, O., Jeuffroy, M. H., Bousset, L.
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Brassica napus; canola; Fundamental and applied biological sciences. Psychology; Fungal plant pathogens; Leptosphaeria maculans; Life Sciences; Phoma; phoma stem canker; Phytopathology and phytopharmacy; Phytopathology. Animal pests. Plant and forest protection; pseudothecial maturation; stem necrosis severity; Vegetal Biology
• ISSN: 0032-0862; 1365-3059
• DOI: 10.1111/j.1365-3059.2008.01931.x

The relationship between severity of blackleg, or phoma stem canker (Leptosphaeria maculans/L. biglobosa), and subsequent primary inoculum production on oilseed rape (Brassica napus) stubble was investigated at two sites in France over 3 years. The quantity of primary inoculum produced in the following year increased with canker severity, from 1·9 to 10·8 pseudothecia cm−2 on stubble with the least and most severe cankers, respectively. Stubble incubated at Le Rheu (cooler, more rain) had 1·7 times more pseudothecia than stubble incubated at Grignon. Stubble collected at Grignon had 2·7 times more pseudothecia than that collected at Le Rheu. The use of Darmor, a cultivar with a good level of quantitative resistance, reduced the severity of canker in the field, but not the subsequent inoculum production for stubble of the same canker severity class. At both sites, maturation of pseudothecia occurred after 63–75 days of incubation and increased with canker severity with a mean of 0·5 and 3% mature pseudothecia appearing per favourable day, on stubble with the least and most severe cankers, respectively. A simplified procedure for pseudothecial quantification proved satisfactory: for all three observers, most (91–96%) of the fructifications counted as pseudothecia were real pseudothecia. Only a few (4–14%) of the fructifications considered as non‐pseudothecia were in fact pseudothecia of L. maculans. The total area occupied by pseudothecia, which was simpler and faster to evaluate, was correlated (coefficient of determination, R2 = 71%) with the number of counted pseudothecia. The results presented here make it possible to forecast the quantity of available primary inoculum for a given disease severity.