Supramolecular Peptide/Surface Assembly for Monitoring Proteinase Activity and Cancer Diagnosis

Matrix metalloproteinases (MMP) are a family of proteolytic enzymes, the expression of which in a key step of tumor progression has recently been better defined. The overexpression of one or more MMPs is thus common among malignant tumors. It may characterize tumor progression and help predict its r...

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Published in:ACS applied materials & interfaces 2015-08, Vol.7 (31), p.16967-16975
Main Authors: Soum, Claire, Rubio-Albenque, Sandra, Fery-Forgues, Suzanne, Déléris, Gérard, Alouini, Mohamed-Anis, Berthelot, Thomas
Format: Article
Language:English
Subjects:
Amino Acid Sequence
beta-Cyclodextrins - chemistry
Cell Line, Tumor
Chemical Sciences
Fluorescent Dyes - chemistry
HeLa Cells
Humans
Kinetics
Lab-On-A-Chip Devices
Matrix Metalloproteinases - metabolism
Microscopy, Fluorescence
Neoplasms - diagnosis
Neoplasms - enzymology
Neoplasms - pathology
Peptides - chemistry
Peptides - metabolism
Severity of Illness Index
Spectrometry, Fluorescence
Surface Properties
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Summary:Matrix metalloproteinases (MMP) are a family of proteolytic enzymes, the expression of which in a key step of tumor progression has recently been better defined. The overexpression of one or more MMPs is thus common among malignant tumors. It may characterize tumor progression and help predict its response to chemotherapy. Consequently, the development of a device for measuring MMP activities is an important challenge for diagnosis and prognosis. In this study, we describe an innovative supramolecular peptide/surface assembly for screening MMP activities. This sensor was used to discriminate various MMP activities and to distinguish between invasive and noninvasive cancerous cell suspensions. Our results confirm the proof-of-concept of a powerful tool for the determination of the tumor aggressiveness and a technical building block for future development of MMP lab-on-chip devices.
ISSN:1944-8244
1944-8252
DOI:10.1021/acsami.5b04871