Inhibition of very long acyl chain sphingolipid synthesis modifies membrane dynamics during plant cytokinesis

Plant cytokinesis requires intense membrane trafficking and remodeling to form a specific membrane structure, the cell plate that will ultimately separate the daughter cells. The nature and the role of lipids involved in the formation of the cell plate remain unclear. Plant membranes are particularl...

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Bibliographic Details
Published in:Biochimica et biophysica acta 2014-10, Vol.1841 (10), p.1422-1430
Main Authors: Molino, Diana, Van der Giessen, Elisabeth, Gissot, Lionel, Hématy, Kian, Marion, Jessica, Barthelemy, Julien, Bellec, Yannick, Vernhettes, Samantha, Satiat-Jeunemaître, Béatrice, Galli, Thierry, Tareste, David, Faure, Jean Denis
Format: Article
Language:English
Subjects:
Biotechnology
Cell plate
Fatty acyl chain length
Life Sciences
Membrane trafficking
Sphingolipid
Vesicle fusion
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Summary:Plant cytokinesis requires intense membrane trafficking and remodeling to form a specific membrane structure, the cell plate that will ultimately separate the daughter cells. The nature and the role of lipids involved in the formation of the cell plate remain unclear. Plant membranes are particularly rich in sphingolipids such as glucosyl-ceramides with long (16 carbons) or very long (24 carbons) acyl chains. We reveal here that inhibition of the synthesis of sphingolipids with very long acyl chains induces defective cell plates with persistent vesicular structures and large gaps. Golgi-derived vesicles carrying material toward the cell plate display longer vesicle–vesicle contact time and their cargos accumulate at the cell plate, suggesting membrane fusion and/or recycling defects. In vitro fusion experiments between artificial vesicles show that glycosphingolipids with very long acyl chains stimulate lipid bilayer fusion. Therefore we propose that the very long acyl chains of sphingolipids are essential structural determinants for vesicle dynamics and membrane fusion during cytokinesis. •Very long acyl chain sphingolipids (VLCFA-SLs) are involved in plant cytokinesis.•Golgi-derived vesicles accumulate at the cell plate in the absence of VLCFA-SLs.•VLCFA-SLs are required for transient Golgi-derived vesicle–vesicle contact.•Glycosphingolipids with very long acyl chains stimulate lipid bilayer fusion.
ISSN:1388-1981
0006-3002
1879-2618
DOI:10.1016/j.bbalip.2014.06.014