Roles of multiple acyl-CoA oxidases in the routing of carbon flow towards β-oxidation and polyhydroxyalkanoate biosynthesis in Yarrowia lipolytica

The oleaginous yeast Yarrowia lipolytica possesses six acyl-CoA oxidase (Aox) isoenzymes encoded by genes POX1-POX6. The respective roles of these multiple Aox isoenzymes were studied in recombinant Y. lipolytica strains that express heterologous polyhydroxyalkanoate (PHA) synthase (phaC) of Pseudom...

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Bibliographic Details
Published in:FEMS yeast research 2010-11, Vol.10 (7), p.917-927
Main Authors: Haddouche, Ramdane, Delessert, Syndie, Sabirova, Julia, Neuvéglise, Cécile, Poirier, Yves, Nicaud, Jean-Marc
Format: Article
Language:English
Subjects:
Acyl-CoA oxidase
Acyl-CoA Oxidase - metabolism
Acyltransferases - genetics
Acyltransferases - metabolism
Biosynthesis
Carbon - metabolism
Fatty acids
Fatty Acids - metabolism
Genotypes
Isoenzymes
Life Sciences
Oxidation
Oxidation-Reduction
PHA
polyhydroxyalkanoate
Polyhydroxyalkanoates - metabolism
Polyhydroxyalkanoic acid
Pseudomonas aeruginosa - enzymology
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Yarrowia - enzymology
Yarrowia - growth & development
Yarrowia - metabolism
Yarrowia lipolytica
yeast
yeasts
β-oxidation
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Summary:The oleaginous yeast Yarrowia lipolytica possesses six acyl-CoA oxidase (Aox) isoenzymes encoded by genes POX1-POX6. The respective roles of these multiple Aox isoenzymes were studied in recombinant Y. lipolytica strains that express heterologous polyhydroxyalkanoate (PHA) synthase (phaC) of Pseudomonas aeruginosa in varying POX genetic backgrounds, thus allowing assessment of the impact of specific Aox enzymes on the routing of carbon flow to β-oxidation or to PHA biosynthesis. Analysis of PHA production yields during growth on fatty acids with different chain lengths has revealed that the POX genotype significantly affects the PHA levels, but not the monomer composition of PHA. Aox3p function was found to be responsible for 90% and 75% of the total PHA produced from either C9:0 or C13:0 fatty acid, respectively, whereas Aox5p encodes the main Aox involved in the biosynthesis of 70% of PHA from C9:0 fatty acid. Other Aoxs, such as Aox1p, Aox2p, Aox4p and Aox6p, were not found to play a significant role in PHA biosynthesis, independent of the chain length of the fatty acid used. Finally, three known models of β-oxidation are discussed and it is shown that a 'leaky-hose pipe model' of the cycle can be applied to Y. lipolytica.
ISSN:1567-1356
1567-1364
DOI:10.1111/j.1567-1364.2010.00670.x