Development of novel enzyme potentiometric biosensor based on pH-sensitive field-effect transistors for aflatoxin B1 analysis in real samples

This study aimed at the development and optimization of a potentiometric biosensor based on pH-sensitive field-effect transistors and acetylcholinesterase for aflatoxin B1 determination in real samples. Optimal conditions for bioselective elements operation were defined and analytical characteristic...

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Bibliographic Details
Published in:Talanta (Oxford) 2015-11, Vol.144, p.1079-1084
Main Authors: Stepurska, K.V., Soldatkin, O.O., Arkhypova, V.M., Soldatkin, A.P., Lagarde, F., Jaffrezic-Renault, N., Dzyadevych, S.V.
Format: Article
Language:English
Subjects:
Acetylcholinesterase
Acetylcholinesterase - metabolism
Aflatoxin B1
Aflatoxin B1 - analysis
Analytical chemistry
Animals
Biosensing Techniques - instrumentation
Biosensor
Chemical Sciences
Hydrogen-Ion Concentration
Inhibitory analysis
Other
pH-sensitive field-effect transistors
Potentiometry
Real samples
Transistors, Electronic
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Summary:This study aimed at the development and optimization of a potentiometric biosensor based on pH-sensitive field-effect transistors and acetylcholinesterase for aflatoxin B1 determination in real samples. Optimal conditions for bioselective elements operation were defined and analytical characteristics of the proposed biosensor were studied. The proposed biosensor characterized high operational stability and reproducibility of signal. Selectivity of acetylcholinesterase-biosensor to aflatoxins in relation to other groups of toxic substances was analyzed. The developed biosensor was applied to the determination of aflatoxin B1 in real samples (sesame, walnut and pea). [Display omitted] •The new potentiometric biosensor for aflatoxin B1 analysis was created.•The main analytical characteristics of the developed biosensor were studied.•The optimal conditions of biosensor work with real samples were defined.•The developed biosensor was applied for aflatoxin B1 analysis in real samples.
ISSN:0039-9140
1873-3573
DOI:10.1016/j.talanta.2015.07.068