Proteomic Approach to Identify Nuclear Proteins in Wheat Grain

The nuclear proteome of the grain of the two cultivated wheat species Triticum aestivum (hexaploid wheat; genomes A, B, and D) and T. monococcum (diploid wheat; genome A) was analyzed in two early stages of development using shotgun-based proteomics. A procedure was optimized to purify nuclei, and a...

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Bibliographic Details
Published in:Journal of proteome research 2015-10, Vol.14 (10), p.4432-4439
Main Authors: Bancel, Emmanuelle, Bonnot, Titouan, Davanture, Marlène, Branlard, Gérard, Zivy, Michel, Martre, Pierre
Format: Article
Language:English
Subjects:
Cell Nucleus - chemistry
Cell Nucleus - genetics
Cell Nucleus - metabolism
Chromatography, Liquid
Edible Grain - chemistry
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
Genome, Plant
Life Sciences
Mass Spectrometry
Metabolic Networks and Pathways - genetics
Molecular Sequence Annotation
Nuclear Proteins - isolation & purification
Plant Proteins - isolation & purification
Proteome - genetics
Proteome - isolation & purification
Proteome - metabolism
Proteomics
Species Specificity
Triticum - classification
Triticum - genetics
Triticum - growth & development
Triticum - metabolism
Vegetal Biology
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Summary:The nuclear proteome of the grain of the two cultivated wheat species Triticum aestivum (hexaploid wheat; genomes A, B, and D) and T. monococcum (diploid wheat; genome A) was analyzed in two early stages of development using shotgun-based proteomics. A procedure was optimized to purify nuclei, and an improved protein sample preparation was developed to efficiently remove nonprotein substances (starch and nucleic acids). A total of 797 proteins corresponding to 528 unique proteins were identified, 36% of which were classified in functional groups related to DNA and RNA metabolism. A large number (107 proteins) of unknown functions and hypothetical proteins were also found. Some identified proteins may be multifunctional and may present multiple localizations. On the basis of the MS/MS analysis, 368 proteins were present in the two species, and in two stages of development, some qualitative differences between species and stages of development were also found. All of these data illustrate the dynamic function of the grain nucleus in the early stages of development.
ISSN:1535-3893
1535-3907
DOI:10.1021/acs.jproteome.5b00446