T Regulatory Lymphocytes Prevent Aldosterone-Induced Vascular Injury

• Published in: Hypertension (Dallas, Tex. 1979) Tex. 1979), 2012-02, Vol.59 (2), p.324-330
• Main Authors: Kasal, Daniel A, Barhoumi, Tlili, Li, Melissa W, Yamamoto, Naoki, Zdanovich, Evguenia, Rehman, Asia, Neves, Mario F, Laurant, Pascal, Paradis, Pierre, Schiffrin, Ernesto L
• Format: Article
• Language: English
• Subjects: Adaptive Immunity; Adoptive Transfer; Aldosterone - adverse effects; Aldosterone - pharmacology; Animals; Arterial hypertension. Arterial hypotension; Biological and medical sciences; Blood and lymphatic vessels; Blood vessels and receptors; Cardiology. Vascular system; Endothelium, Vascular - drug effects; Endothelium, Vascular - metabolism; Endothelium, Vascular - physiopathology; Fundamental and applied biological sciences. Psychology; Hypertension - chemically induced; Hypertension - prevention & control; Immunity, Innate; Life Sciences; Male; Medical sciences; Mesenteric Arteries - drug effects; Mesenteric Arteries - metabolism; Mesenteric Arteries - physiopathology; Mice; Mice, Inbred C57BL; Models, Animal; NADPH Oxidases - metabolism; Renal Artery - drug effects; Renal Artery - metabolism; Renal Artery - physiopathology; Superoxides - metabolism; T-Lymphocytes, Regulatory - cytology; T-Lymphocytes, Regulatory - physiology; Vasculitis - chemically induced; Vasculitis - prevention & control; Vertebrates: cardiovascular system
• ISSN: 0194-911X; 1524-4563
• DOI: 10.1161/HYPERTENSIONAHA.111.181123

Aldosterone mediates actions of the renin-angiotensin-aldosterone system inducing hypertension, oxidative stress, and vascular inflammation. Recently, we showed that angiotensin II–induced hypertension and vascular damage are mediated at least in part by macrophages and T-helper effector lymphocytes. Adoptive transfer of suppressor T-regulatory lymphocytes (Tregs) prevented angiotensin II action. We hypothesized that Treg adoptive transfer would blunt aldosterone-induced hypertension and vascular damage. Thirteen to 15-week–old male C57BL/6 mice were injected intravenously at 1-week intervals with 3×10 CD4CD25 cells (representing Treg) or control CD4CD25 cells and then infused or not for 14 days with aldosterone (600 μg/kg per day, SC) while receiving 1% saline to drink. Aldosterone induced a small but sustained increase in blood pressure (P