Neurofilaments and NFL-TBS.40–63 peptide penetrate oligodendrocytes through clathrin-dependent endocytosis to promote their growth and survival in vitro

Highlights • Endocytosis of neurofilaments (NF), tubulin (TUB), and NFL-TBS.40–63 peptide by oligodendrocyte (OL) is evidenced in vitro. • NFL-TBS.40–63 endocytosis is mainly specific, as a scrambled NFL peptide is not significantly uptaken. • Inhibition by dynasore suggests a clathrin-dependent mec...

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Bibliographic Details
Published in:Neuroscience 2015-07, Vol.298, p.42-51
Main Authors: Fressinaud, C, Eyer, J
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn
axon–glia interactions
Biotinylation
Brain - cytology
Cell Differentiation - drug effects
Cell Survival - drug effects
Cells, Cultured
Clathrin - metabolism
Culture Media, Conditioned - pharmacology
demyelination
Dose-Response Relationship, Drug
Endocytosis - drug effects
Endocytosis - physiology
Intermediate Filaments - metabolism
Keratins - metabolism
Life Sciences
microtubule
multiple sclerosis
Myelin Basic Protein - metabolism
Neurofilament Proteins - metabolism
Neurology
Oligodendroglia - drug effects
Oligodendroglia - metabolism
Peptide Fragments - metabolism
Peptides - pharmacology
Rats
Rats, Sprague-Dawley
Tubulin - metabolism
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Summary:Highlights • Endocytosis of neurofilaments (NF), tubulin (TUB), and NFL-TBS.40–63 peptide by oligodendrocyte (OL) is evidenced in vitro. • NFL-TBS.40–63 endocytosis is mainly specific, as a scrambled NFL peptide is not significantly uptaken. • Inhibition by dynasore suggests a clathrin-dependent mechanism for NF and NFL-TBS.40–63 peptide, but not TUB. • Endocytosis by OL of NF released during multiple sclerosis might promote remyelination.
ISSN:0306-4522
1873-7544
DOI:10.1016/j.neuroscience.2015.04.003