Use of Solanum stenotomum for introduction of resistance to bacterial wilt in somatic hybrids of potato

The wild tuber-bearing relative Solanum stenotomum was used as source of resistance to bacterial wilt caused by Ralstonia solanacearum. In order to transfer resistance, somatic hybrids between a dihaploid clone of potato ( S. tuberosum) cv. BF15 and S. stenotomum were produced by electrofusion of me...

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Bibliographic Details
Published in:Plant physiology and biochemistry 2001-10, Vol.39 (10), p.899-908
Main Authors: Fock, Isabelle, Collonnier, Cécile, Luisetti, Jacques, Purwito, Agus, Souvannavong, Vongthip, Vedel, Fernand, Servaes, Aline, Ambroise, Annick, Kodja, Hippolyte, Ducreux, Georges, Sihachakr, Darasinh
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
bacterial wilt
Biological and medical sciences
DISEASE RESISTANCE
electrofusion
ENFERMEDADES DE LAS PLANTAS
Fundamental and applied biological sciences. Psychology
Genetics and breeding of economic plants
GENOTIPOS
GENOTYPE
GENOTYPES
HIBRIDOS SOMATICOS
HORTALIZAS (PLANTAS)
HYBRIDE SOMATIQUE
Life Sciences
MALADIE DES PLANTES
Pest resistance
PLANT DISEASES
Plant pathogens
PLANTE LEGUMIERE
RALSTONIA SOLANACEARUM
RESISTANCE AUX MALADIES
RESISTENCIA A LA ENFERMEDAD
S. tuberosum
SOLANUM
Solanum stenotomum
SOLANUM TUBEROSUM
SOMATIC HYBRIDS
Varietal selection. Specialized plant breeding, plant breeding aims
VEGETABLE CROPS
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Summary:The wild tuber-bearing relative Solanum stenotomum was used as source of resistance to bacterial wilt caused by Ralstonia solanacearum. In order to transfer resistance, somatic hybrids between a dihaploid clone of potato ( S. tuberosum) cv. BF15 and S. stenotomum were produced by electrofusion of mesophyll protoplasts. A total of thirty hybrid plants were regenerated. When transferred to the greenhouse, they exhibited a strong vigour and showed morphological intermediate traits, including leaf form, flowers and tuber characteristics. DNA analysis using flow cytometry revealed that 25 were tetraploids (4×; 48 chromosomes), three hexaploids (6×; 72) and two aneuploids (
ISSN:0981-9428
1873-2690
DOI:10.1016/S0981-9428(01)01307-9