Mass spectrometry for pectin structure analysis

Pectin are extremely complex biopolymers made up of different structural domains. Enzymatic degradation followed by purification and structural analysis of the degradation products proved to be efficient tools for the understanding of pectin fine structure, including covalent interactions between pe...

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Bibliographic Details
Published in:Carbohydrate research 2009-09, Vol.344 (14), p.1798-1807
Main Authors: Ralet, Marie-Christine, Lerouge, Patrice, Quéméner, Bernard
Format: Article
Language:English
Subjects:
Acetylation
Cell wall polysaccharides
Homogalacturonan
Life Sciences
Mass Spectrometry
Methylation
Pectic oligosaccharides
Pectins - chemistry
Pectins - ultrastructure
Rhamnogalacturonan
Spectrometry, Mass, Electrospray Ionization
Triiodothyronine - analogs & derivatives
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Summary:Pectin are extremely complex biopolymers made up of different structural domains. Enzymatic degradation followed by purification and structural analysis of the degradation products proved to be efficient tools for the understanding of pectin fine structure, including covalent interactions between pectic structural domains or with other cell wall polysaccharides. Due to its high sensitivity, high throughput and capacity to analyze mixtures, mass spectrometry has gained more and more importance as a tool for oligosaccharides structural characterization in the past 10 years. This review will focus on the combined use of mass spectrometry and enzymatic digestion for pectins structural characterization.
ISSN:0008-6215
1873-426X
0008-6215
DOI:10.1016/j.carres.2008.08.036