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Transcription profiling of the chilling requirement for bud break in apples: a putative role for FLC-like genes
Apple production depends on the fulfilment of a chilling requirement for bud dormancy release. Insufficient winter chilling results in irregular and suboptimal bud break in the spring, with negative impacts on apple yield. Trees from apple cultivars with contrasting chilling requirements for bud bre...
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Published in: | Journal of experimental botany 2015-05, Vol.66 (9), p.2659-2672 |
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description | Apple production depends on the fulfilment of a chilling requirement for bud dormancy release. Insufficient winter chilling results in irregular and suboptimal bud break in the spring, with negative impacts on apple yield. Trees from apple cultivars with contrasting chilling requirements for bud break were used to investigate the expression of the entire set of apple genes in response to chilling accumulation in the field and controlled conditions. Total RNA was analysed on the AryANE v.1.0 oligonucleotide microarray chip representing 57 000 apple genes. The data were tested for functional enrichment, and differential expression was confirmed by real-time PCR. The largest number of differentially expressed genes was found in samples treated with cold temperatures. Cold exposure mostly repressed expression of transcripts related to photosynthesis, and long-term cold exposure repressed flavonoid biosynthesis genes. Among the differentially expressed selected candidates, we identified genes whose annotations were related to the circadian clock, hormonal signalling, regulation of growth, and flower development. Two genes, annotated as FLOWERING LOCUS C-like and MADS AFFECTING FLOWERING, showed strong differential expression in several comparisons. One of these two genes was upregulated in most comparisons involving dormancy release, and this gene’s chromosomal position co-localized with the confidence interval of a major quantitative trait locus for the timing of bud break. These results indicate that photosynthesis and auxin transport are major regulatory nodes of apple dormancy and unveil strong candidates for the control of bud dormancy. |
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Insufficient winter chilling results in irregular and suboptimal bud break in the spring, with negative impacts on apple yield. Trees from apple cultivars with contrasting chilling requirements for bud break were used to investigate the expression of the entire set of apple genes in response to chilling accumulation in the field and controlled conditions. Total RNA was analysed on the AryANE v.1.0 oligonucleotide microarray chip representing 57 000 apple genes. The data were tested for functional enrichment, and differential expression was confirmed by real-time PCR. The largest number of differentially expressed genes was found in samples treated with cold temperatures. Cold exposure mostly repressed expression of transcripts related to photosynthesis, and long-term cold exposure repressed flavonoid biosynthesis genes. Among the differentially expressed selected candidates, we identified genes whose annotations were related to the circadian clock, hormonal signalling, regulation of growth, and flower development. Two genes, annotated as FLOWERING LOCUS C-like and MADS AFFECTING FLOWERING, showed strong differential expression in several comparisons. One of these two genes was upregulated in most comparisons involving dormancy release, and this gene’s chromosomal position co-localized with the confidence interval of a major quantitative trait locus for the timing of bud break. These results indicate that photosynthesis and auxin transport are major regulatory nodes of apple dormancy and unveil strong candidates for the control of bud dormancy.</description><identifier>ISSN: 0022-0957</identifier><identifier>EISSN: 1460-2431</identifier><identifier>DOI: 10.1093/jxb/erv061</identifier><identifier>PMID: 25750421</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Agricultural sciences ; Circadian Clocks ; Cluster Analysis ; Cold Temperature ; Flavonoids - biosynthesis ; Flowers - genetics ; Flowers - growth & development ; Gene Expression Profiling ; Gene Expression Regulation, Plant ; Genes, Plant ; Life Sciences ; Malus - genetics ; Malus - growth & development ; Oligonucleotide Array Sequence Analysis ; Quantitative Trait Loci ; RESEARCH PAPER ; Seasons ; Signal Transduction</subject><ispartof>Journal of experimental botany, 2015-05, Vol.66 (9), p.2659-2672</ispartof><rights>The Author 2015</rights><rights>The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c445t-a786f3b4ea20ef487d264b0981c4c7422f580c2e0fae744897554d6e2044687a3</citedby><cites>FETCH-LOGICAL-c445t-a786f3b4ea20ef487d264b0981c4c7422f580c2e0fae744897554d6e2044687a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/26390100$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/26390100$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,780,784,885,27924,27925,58238,58471</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25750421$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-01455987$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Porto, Diogo Denardi</creatorcontrib><creatorcontrib>Bruneau, Maryline</creatorcontrib><creatorcontrib>Perini, Pâmela</creatorcontrib><creatorcontrib>Anzanello, Rafael</creatorcontrib><creatorcontrib>Renou, Jean-Pierre</creatorcontrib><creatorcontrib>dos Santos, Henrique Pessoa</creatorcontrib><creatorcontrib>Fialho, Flávio Bello</creatorcontrib><creatorcontrib>Revers, Luís Fernando</creatorcontrib><title>Transcription profiling of the chilling requirement for bud break in apples: a putative role for FLC-like genes</title><title>Journal of experimental botany</title><addtitle>J Exp Bot</addtitle><description>Apple production depends on the fulfilment of a chilling requirement for bud dormancy release. Insufficient winter chilling results in irregular and suboptimal bud break in the spring, with negative impacts on apple yield. Trees from apple cultivars with contrasting chilling requirements for bud break were used to investigate the expression of the entire set of apple genes in response to chilling accumulation in the field and controlled conditions. Total RNA was analysed on the AryANE v.1.0 oligonucleotide microarray chip representing 57 000 apple genes. The data were tested for functional enrichment, and differential expression was confirmed by real-time PCR. The largest number of differentially expressed genes was found in samples treated with cold temperatures. Cold exposure mostly repressed expression of transcripts related to photosynthesis, and long-term cold exposure repressed flavonoid biosynthesis genes. Among the differentially expressed selected candidates, we identified genes whose annotations were related to the circadian clock, hormonal signalling, regulation of growth, and flower development. Two genes, annotated as FLOWERING LOCUS C-like and MADS AFFECTING FLOWERING, showed strong differential expression in several comparisons. One of these two genes was upregulated in most comparisons involving dormancy release, and this gene’s chromosomal position co-localized with the confidence interval of a major quantitative trait locus for the timing of bud break. These results indicate that photosynthesis and auxin transport are major regulatory nodes of apple dormancy and unveil strong candidates for the control of bud dormancy.</description><subject>Agricultural sciences</subject><subject>Circadian Clocks</subject><subject>Cluster Analysis</subject><subject>Cold Temperature</subject><subject>Flavonoids - biosynthesis</subject><subject>Flowers - genetics</subject><subject>Flowers - growth & development</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes, Plant</subject><subject>Life Sciences</subject><subject>Malus - genetics</subject><subject>Malus - growth & development</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Quantitative Trait Loci</subject><subject>RESEARCH PAPER</subject><subject>Seasons</subject><subject>Signal Transduction</subject><issn>0022-0957</issn><issn>1460-2431</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNpF0MtLxDAQBvAgiq6Pi3clRxXqTtI82qOIL1zwoueQdidu1m5bk1b0v7drVz2Fyfz4YD5CjhlcMsjT6fKzmGL4AMW2yIQJBQkXKdsmEwDOE8il3iP7MS4BQIKUu2SPSy1BcDYhj8_B1rEMvu18U9M2NM5Xvn6ljaPdAmm58NXPHPC99wFXWHfUNYEW_ZwWAe0b9TW1bVthPCQ7zlYRjzbvAXm5vXm-vk9mT3cP11ezpBRCdonVmXJpIdByQCcyPedKFJBnrBSlFpw7mUHJEZxFLUSWaynFXCEHIVSmbXpAzsfcha1MG_zKhi_TWG_ur2Zm_QdMSJln-oMN9my0w2XvPcbOrHwssapsjU0fDVNap5qrdE0vRlqGJsaA7i-bgVkXbYaizVj0gE83uX2xwvkf_W12ACcjWMauCf97lebAANJvgwCCBA</recordid><startdate>20150501</startdate><enddate>20150501</enddate><creator>Porto, Diogo Denardi</creator><creator>Bruneau, Maryline</creator><creator>Perini, Pâmela</creator><creator>Anzanello, Rafael</creator><creator>Renou, Jean-Pierre</creator><creator>dos Santos, Henrique Pessoa</creator><creator>Fialho, Flávio Bello</creator><creator>Revers, Luís Fernando</creator><general>Oxford University Press</general><general>Oxford University Press (OUP)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope></search><sort><creationdate>20150501</creationdate><title>Transcription profiling of the chilling requirement for bud break in apples</title><author>Porto, Diogo Denardi ; 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Insufficient winter chilling results in irregular and suboptimal bud break in the spring, with negative impacts on apple yield. Trees from apple cultivars with contrasting chilling requirements for bud break were used to investigate the expression of the entire set of apple genes in response to chilling accumulation in the field and controlled conditions. Total RNA was analysed on the AryANE v.1.0 oligonucleotide microarray chip representing 57 000 apple genes. The data were tested for functional enrichment, and differential expression was confirmed by real-time PCR. The largest number of differentially expressed genes was found in samples treated with cold temperatures. Cold exposure mostly repressed expression of transcripts related to photosynthesis, and long-term cold exposure repressed flavonoid biosynthesis genes. Among the differentially expressed selected candidates, we identified genes whose annotations were related to the circadian clock, hormonal signalling, regulation of growth, and flower development. Two genes, annotated as FLOWERING LOCUS C-like and MADS AFFECTING FLOWERING, showed strong differential expression in several comparisons. One of these two genes was upregulated in most comparisons involving dormancy release, and this gene’s chromosomal position co-localized with the confidence interval of a major quantitative trait locus for the timing of bud break. These results indicate that photosynthesis and auxin transport are major regulatory nodes of apple dormancy and unveil strong candidates for the control of bud dormancy.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>25750421</pmid><doi>10.1093/jxb/erv061</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Agricultural sciences Circadian Clocks Cluster Analysis Cold Temperature Flavonoids - biosynthesis Flowers - genetics Flowers - growth & development Gene Expression Profiling Gene Expression Regulation, Plant Genes, Plant Life Sciences Malus - genetics Malus - growth & development Oligonucleotide Array Sequence Analysis Quantitative Trait Loci RESEARCH PAPER Seasons Signal Transduction |
title | Transcription profiling of the chilling requirement for bud break in apples: a putative role for FLC-like genes |
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