Deregulated expression of VHL mRNA variants in papillary thyroid cancer

Recent findings demonstrated that a subset of papillary thyroid cancers (PTCs) is characterized by reduced expression of the von Hippel-Lindau (VHL) tumor suppressor gene, and that lowest levels associated with more aggressive PTCs. In the present study, the levels of the two VHL mRNA splicing varia...

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Published in:Molecular and cellular endocrinology 2017-03, Vol.443, p.121-127
Main Authors: Baldini, Enke, Tuccilli, Chiara, Arlot-Bonnemains, Yannick, Chesnel, Frank, Sorrenti, Salvatore, De Vito, Corrado, Catania, Antonio, D'Armiento, Eleonora, Antonelli, Alessandro, Fallahi, Poupak, Watutantrige-Fernando, Sara, Tartaglia, Francesco, Barollo, Susi, Mian, Caterina, Bononi, Marco, Arceri, Stefano, Mascagni, Domenico, Vergine, Massimo, Pironi, Daniele, Monti, Massimo, Filippini, Angelo, Ulisse, Salvatore
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Aged, 80 and over
Alternative Splicing - genetics
Biomarkers
Cancer
Carcinoma, Papillary - genetics
Carcinoma, Papillary - pathology
Child
Disease-Free Survival
Female
Gene expression
Gene Expression Regulation, Neoplastic
Genetics
Humans
Kaplan-Meier Estimate
Life Sciences
Male
Middle Aged
Mutation - genetics
Papillary thyroid cancer
Prognosis
Proto-Oncogene Proteins B-raf - genetics
RNA, Messenger - genetics
RNA, Messenger - metabolism
Thyroid Cancer, Papillary
Thyroid Neoplasms - genetics
Thyroid Neoplasms - pathology
VHL
Von Hippel-Lindau Tumor Suppressor Protein - genetics
Von Hippel-Lindau Tumor Suppressor Protein - metabolism
Young Adult
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Summary:Recent findings demonstrated that a subset of papillary thyroid cancers (PTCs) is characterized by reduced expression of the von Hippel-Lindau (VHL) tumor suppressor gene, and that lowest levels associated with more aggressive PTCs. In the present study, the levels of the two VHL mRNA splicing variants, VHL-213 (V1) and VHL-172 (V2), were measured in a series of 96 PTC and corresponding normal matched tissues by means of quantitative RT-PCR. Variations in the mRNA levels were correlated with patients' clinicopathological parameters and disease-free interval (DFI). The analysis of VHL mRNA in tumor tissues, compared to normal matched tissues, revealed that its expression was either up- or down-regulated in the majority of PTC. In particular, V1 and V2 mRNA levels were altered, respectively, in 78 (81.3%) and 65 (67.7%) out of the 96 PTCs analyzed. A significant positive correlation between the two mRNA variants was observed (p 
ISSN:0303-7207
1872-8057
0303-7207
DOI:10.1016/j.mce.2017.01.019