Comparison of α-acetolactate synthase and α-acetolactate decarboxylase in Lactococcus spp. and Leuconostoc spp

Cell-free extracts of Leuconostoc and Lactococcus species were tested for their alpha -acetolactate synthase and alpha -acetolactate decarboxylase activities. In Leuconostoc mesenteroides subsp. cremoris, Leuconostoc mesenteroides subsp. mesenteroides and Leuconostoc lactis, the Km of alpha -acetola...

Full description

Saved in:
Bibliographic Details
Published in:Biotechnology letters 1994-01, Vol.16 (3), p.257-262
Main Authors: MONNET, C, PHALIP, V, SCHMITT, P, DIVIES, C
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Enzyme engineering
Fundamental and applied biological sciences. Psychology
Lactococcus lactis lactis
Leuconostoc lactis
Leuconostoc mesenteroides mesenteroides
Life Sciences
Methods. Procedures. Technologies
Miscellaneous
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cell-free extracts of Leuconostoc and Lactococcus species were tested for their alpha -acetolactate synthase and alpha -acetolactate decarboxylase activities. In Leuconostoc mesenteroides subsp. cremoris, Leuconostoc mesenteroides subsp. mesenteroides and Leuconostoc lactis, the Km of alpha -acetolactate synthase for pyruvate was close to 10 mM whereas it was 30 mM in Lactococcus lactis subsp. lactis biovar. diacetylactis. The Km of alpha -acetolactate decarboxylase for alpha -acetolactic acid was very low (0.3 mM) in Leuconostoc species in comparison to Lactococcus lactis subsp. lactis biovar. diacetylactis (60 mM). In the latter bacterium, alpha -acetolactate decarboxylase showed a sigmoidal dependence upon alpha -acetolactic acid and was activated by the three branched-chain amino acids: leucine, isoleucine and valine.
ISSN:0141-5492
1573-6776
DOI:10.1007/BF00134622