Certification of methylmercury content in two fresh-frozen reference materials: SRM 1947 Lake Michigan fish tissue and SRM 1974b organics in mussel tissue (Mytilus edulis)

This paper describes the development of two independent analytical methods for the extraction and quantification of methylmercury from marine biota. The procedures involve microwave extraction, followed by derivatization and either headspace solid-phase microextraction (SPME) with a polydimethylsilo...

Full description

Saved in:
Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2007-04, Vol.387 (7), p.2335-2341
Main Authors: Davis, W Clay, Christopher, S J, Pugh, Rebecca S, Donard, O F X, Krupp, Eva A, Point, David, Horvat, Milena, Gibicar, D, Kljakovic-Gaspic, Z, Porter, Barbara J, Schantz, Michele M
Format: Article
Language:English
Subjects:
Analytical chemistry
Animals
Chemical Sciences
Chromatography, Gas - methods
Fishes
Food Contamination
Freezing
Gas Chromatography-Mass Spectrometry - methods
Meat - analysis
Methylmercury Compounds - analysis
Mytilus edulis - chemistry
Reference Values
Seafood - analysis
Solid Phase Microextraction - methods
Trace Elements - analysis
United States
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This paper describes the development of two independent analytical methods for the extraction and quantification of methylmercury from marine biota. The procedures involve microwave extraction, followed by derivatization and either headspace solid-phase microextraction (SPME) with a polydimethylsiloxane (PDMS)-coated silica fiber or back-extraction into iso-octane. The identification and quantification of the extracted compounds is carried out by capillary gas chromatography/mass spectrometric (GC/MS) and inductively coupled plasma mass spectrometric (GC/ICP-MS) detection. Both methods were validated for the determination of methylmercury (MeHg) concentrations in a variety of biological standard reference materials (SRMs) including fresh-frozen tissue homogenates of SRM 1946 Lake Superior fish tissue and SRM 1974a organics in mussel tissue (Mytilus edulis) and then applied to the certification effort of SRM 1947 Lake Michigan fish tissue and SRM 1974b organics in mussel tissue (Mytilus edulis). While past certifications of methylmercury in tissue SRMs have been based on two independent methods from the National Institute of Standards and Technology (NIST) and participating laboratories, the methods described within provide improved protocols and will allow future certification efforts to be based on at least two independent analytical methods within NIST.
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-006-1106-x