MALDI-TOF and 13C NMR analysis of Tunisian Zizyphus jujuba root bark tannins

•MALDI mass spectrometry was used to determine oligomer types and distribution of jubjube (Zyziphus jujuba) root bark tannin extract.•Mixed flavonoid oligomers, up to hexamers were found. Fisetinidin, robinetinidin, catechin and gallocatechin units are present.•The flavonoid oligomers were frequentl...

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Bibliographic Details
Published in:Industrial crops and products 2014-08, Vol.59, p.277-281
Main Authors: Abdalla, S., Pizzi, A., Ayed, N., Charrier, F., Bahabri, F., Ganash, A.
Format: Article
Language:English
Subjects:
Engineering Sciences
Jubjube
MALDI
NMR
Oligomers distribution
Tannin
Zyziphus jujuba
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Summary:•MALDI mass spectrometry was used to determine oligomer types and distribution of jubjube (Zyziphus jujuba) root bark tannin extract.•Mixed flavonoid oligomers, up to hexamers were found. Fisetinidin, robinetinidin, catechin and gallocatechin units are present.•The flavonoid oligomers were frequently covalently linked to carbohydrate monomers and dimers.•The site of the carbohydrates linkage to the flavonoid has been identified by 13C NMR spectroscopy. Matrix assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry was used to examine the oligomer types and their distribution of jubjube (Zyziphus jujuba Mill.) root bark tannin extract. The flavonoid oligomers, up to hexamers, of lower degree of polymerization than that of commercial tannins, are very frequently covalently linked to carbohydrate monomers and dimers. The site of carbohydrate linkage to the flavonoid has been identified by 13C NMR spectroscopy. Fisetinidin, robinetinidin, catechin and gallocatechin flavonoid units are all present in this tannin, all entering to form mixed oligomers based on these four units. This forbids the classification of this extract as just a simple procyanidin, prorobinetinidin, profisetinidin or prodelphinidin.
ISSN:0926-6690
1872-633X
DOI:10.1016/j.indcrop.2014.05.035